Fig. 5

Deletion of PDK1 in osteoclasts inhibited RANKL‐stimulated expression of osteoclast-specific gene and NF‐κB activities. A Relative mRNA expression levels of Ctsk, TRAP, MMP-9, and NFATc1; mRNA expression was normalized to WT mice. B BMMs were stimulated with RANKL for 0, 5, 10, 30, or 60 min; total cell proteins (TCPs) were obtained and the expression of p-AKT, AKT, P65, p-P65, IκBα, and p-IκBα was detected by Western blot. C, E, F Relative ratios of phosphorylated/unphosphorylated proteins. D BMMs were triggered with RANKL for 0, 2, 4, or 6 days; TCPs were extracted and the expressions of p-AKT, AKT, P65, p-P65, IκBα, and p-IκBα were identified by Western blot. G, H, I, J Relative expression of PDK1, Ctsk, RANK, and NFATc1. N = 3, all data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. BMMs, bone marrow-derived macrophage cells; PDK1, 3-phosphoinositide-dependent protein kinase-1; Ctsk, cathepsin K; NFATc1, nuclear factor of activated T cells; RANK, receptor activator for nuclear factor-κB