Fig. 4
From: Extracellular vesicles derived from endothelial cells modulate macrophage phenotype in vitro
iNOS expression in and CD86 expression on NR8383 macrophages upon 24 h stimulation with E-EVs. A Immunofluorescence analysis of the iNOS expression in NR8383 macrophages after stimulation with either plain PBS, ELPS-EVs, or ENor-EVs. iNOS (green fluorescence) was detected in ELPS-EVs treated cells, while it could not be concluded in PBS nor ENor-EVs treated cells. DAPI (blue fluorescence) indicates cellular nuclei. Used magnification was × 400. n = 3 replicates were used for these experiments. *p < 0.0001 for ELPS-EVs compared with the PBS and ENor-EVs groups. Statistical analysis was performed by one‐way ANOVA with Bonferroni's correction. Scale bar: 30 µm. B Flow cytometry analysis of CD86 expression on NR8383 macrophages upon stimulation with either plain PBS, ELPS-EVs, or ENor-EVs. CD86 expression significantly increased on NR8383 macrophages as a result of the ELPS-EVs stimulation when compared to the expression levels of the PBS and ENor-EVs groups. CD86 expression quantification is represented as average in % + SEM (n = 3 replicates). *p < 0.0001 for ELPS-EVs compared with the PBS and ENor-EVs groups. Statistical analysis was performed by one‐way ANOVA with Bonferroni's correction