Table 2 Primer sequences used for RT-qPCR
PCR primers | Sequence(s) | Annealing temperature |
|---|---|---|
ESR1-forward | TGGGAATGATGAAAGGTGGGAT | 53 ℃ |
ESR1-reverse | GGTTGGCAGCTCTCATGTCT | |
PGR-forward | CAGCCAGAGCCCACAATACA | 55 ℃ |
PGR-reverse | GTTGTGCTGCCCTTCCATTG | |
GHR-forward | GCGCAGACGCGAACC | 51 ℃ |
GHR-reverse | AGGCTCCTTAGAAGAATTTGTCTTT | |
FSHR-forward | ATAAGGGCACTGTGTGGAGC | 53 ℃ |
FSHR-reserve | TGGTGAGGACAAACCTCAGTT |