Profiling of the fecal microbiota and circulating microRNA-16 in IBS subjects with Blastocystis infection : a case–control study

Table 1 Targeted microorganism and their primers

Microorganisms	Oligonucleotide sequence (5`–3`)	Product size (bp)	Refs
Eubacteria	ACTCCTACGGGAGGCAGCAGT	200	[25]
Eubacteria	ATTACCGCGGCTGCTGGC	200	[25]
Firmicutes	GGAGYATGTGGTTTAATTCGAAGCA	129	[26]
Firmicutes	AGCTGACGACAACCATGCAC	129	[26]
Bacteroidetes	GTTTAATTCGATGATACGCG	137	[26]
Bacteroidetes	TTAAGCCGACACCTCACG	137	[26]
Actinobacteria	GCGACCTATCAGCTTGTT	345	[27]
Actinobacteria	CCGCCTACGAGCTCTTTACGC	345	[27]
Ruminococcus spp.	GGCGGCYTRCTGGGCTTT	302	[28]
Ruminococcus spp.	CCAGGTGGATWACTTATTGTGTTAA	302	[28]
Prevotella spp.	CACCAAGGCGACGATCA	507	[29]
Prevotella spp.	GGATAACGCCTGGACCT	507	[29]
Methanobacteria spp.	CGATGCGGACTTGGTGTTG	184	[29]
Methanobacteria spp.	TGTCGCCTCTGGTGAGATGTC	184	[29]
Faecalibacterium spp.	GATGGCCTCGCGTCCGATTAG	198	[29]
Faecalibacterium spp.	CCGAAGACCTTCTTCCTCC	198	[29]
Bifidobacteria spp.	GGGATGCTGGTGTGGAAGAG	200	[30]
Bifidobacteria spp.	TGCTCGCGTCCACTATCCAG	200	[30]
Lactobacillus spp.	TGGATGCCTTGGCACTAG	89	[31]
Lactobacillus spp.	AAATCTCCGGATCAAAGCTTAC	89	[31]
Roseburia spp.	GCGGTGCGGCAAGTCTGA	81	[This study]
Roseburia spp.	CCTCCGACACTCTAGTACGAC	81	[This study]
Blautia spp.	GCAAGTCTGATGTGAAAGGCTG	251	[This study]
Blautia spp.	TTGCCACCCGACACCTAGTA	251	[This study]
Alistipes spp.	TTAGAGATGGGCATGCGTTGT	320	[32]
Alistipes spp.	TGAATCCTCCGTATT	320	[32]
Fusobacteria	GATCCAGCAATTCTGTGTG	290	[29]
Fusobacteria	CGAATTTCACCTCTACACTTG	290	[29]
Streptococcus spp.	GTACAGTTGCTTCAGGACGT	195	[27]

ISSN: 2047-783X