Fig. 1

PLAC8 and ALKBH5 expression in placental tissue of PE patients and hypoxia-induced trophoblast cells. The placenta tissue was collected from PE patients (n = 30) and healthy people (HC, n = 30). A, B The mRNA levels of PLAC8 and ALKBH5 in placenta tissue were detected by qRT-PCR. C Protein levels of PLAC8 and ALKBH5 in placenta tissue were detected by western blot. D Relative PLAC8 m6A level in clinical samples was detected. E The correlation between PLAC8 and ALKBH5 expression in clinical tissues was analyzed. The HTR-8/Svneo cells were induced by hypoxia. F The mRNA levels of PLAC8 and ALKBH5 in HTR-8/Svneo cells were detected by qRT-PCR. G Protein levels of PLAC8 and ALKBH5 in HTR-8/Svneo cells were detected by western blot. H Relative PLAC8 m6A level in hypoxia-induced trophoblast cells was detected. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001