Fig. 2

Regulation of PLAC8 on the migration and invasion of trophoblast cells. The HTR-8/SVneo cells were transfected with PLAC8 (or pcDNA). N = 3. A The mRNA levels of PLAC8 in HTR-8/Svneo cells were detected by qRT-PCR. B Protein levels of PLAC8 in HTR-8/Svneo cells. C, D Cell migration and invasion were detected by the Transwell assay. E Protein levels of MMP-2 and MMP-9 in HTR-8/Svneo cells were detected by western blot. F The secretion levels of MMP-2 and MMP-9 in HTR-8/Svneo cells were detected by ELISA. The HTR-8/SVneo cells were transfected with siRNA-PLAC8 (or siRNA-NC) before being treated with hypoxia. G The mRNA levels of PLAC8 in HTR-8/Svneo cells were detected by qRT-PCR. H Protein levels of PLAC8 in HTR-8/Svneo cells. I, K Cell migration and invasion were detected by the Transwell assay. J Protein levels of MMP-2 and MMP-9 in HTR-8/Svneo cells were detected by western blot. L The secretion levels of MMP-2 and MMP-9 in HTR-8/Svneo cells were detected by ELISA. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001