Fig. 4

Effect of ALKBH5 on PLAC8 m6A modification. A RIP assay was performed in HTR-8/SVneo cells. B RNA-pull down assay was performed in HTR-8/SVneo cells. The HTR-8/SVneo cells were transfected with pcDNA-ALKBH5 (or pcDNA-NC). N = 3. C The MeRIP assay was used to detect the PLAC8 m6A level. D, E PLAC8 expression was detected by qRT-PCR and Western blot. F The mRNA levels of PLAC8 in HTR-8/Svneo cells were detected by qRT-PCR. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001