Fig. 4

Effects of Kaempferol on osteogenesis in the presence of 1% Ti-alloy particles after 21 days of osteogenic differentiation. A Calcified matrix and quantification of Alizarin Red staining intensity of cells treated with Kaempferol and Ti-alloy particles following osteogenic differentiation for 21 days. B ALP activity in culture medium treated with Kaempferol and Ti alloy-particles at 7, 14, and 21 days of osteogenic differentiation. C–G Expression of Runx2, OCN, Sp-7 and β-catenin determined by western-blot analysis following treatment with the Kaempferol after 21 days of osteogenic differentiation. H BMSCs were treated with Ti-alloy particles and Kaempferol for 24 h. Cell viability was detected by CCK-8 assay. I RT-PCR of Runx2, ALP, OCN and β-catenin, Lef-1, Tcf-4 after 21 days of osteogenic differentiation. Data are shown as the mean ± SD. Compared with Control group. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, n = 3