Table 2 Primer sequences for ALP, β-catenin, Lef-1, Tcf-4 and GAPDH
Gene | Sequences ( 5′–3′) | Product size (bp) | |
|---|---|---|---|
Runx2 | Forward | CGGGTCTCCTTCCAGGAT | 18 |
Reverse | GGGAACTGCTGTGGCTTC | 18 | |
OCN | Forward | GAGGGCAATAAGGTAGTGAA | 20 |
Reverse | CATAGATGCGTTTGTAGGC | 19 | |
ALP | Forward | CCCAAAGGCTTCTTCTTGC | 19 |
Reverse | GCCTGGTAGTTGTTGTGAG | 19 | |
β-catenin | Forward | ACAGGGTGCTATTCCACGAC | 20 |
Reverse | CTGCACAAACAATGGAATGG | 20 | |
Lef-1 | Forward | GCCACCGATGAGATGATCCC | 20 |
Reverse | TTGATGTCGGCTAAGTCGCC | 20 | |
Tcf-4 | Forward | ATGGCCCAAGTAGTGATGTCT | 21 |
Reverse | CAAACACGTCGGTCTCATACA | 21 | |
GAPDH | Forward | CAATGACCCCTTCATTGACC | 20 |
Reverse | TGGACTCCACGACGTACTCA | 20 |