Fig. 4
From: GREB1L overexpression is associated with good clinical outcomes in breast cancer
Clinical relevance of GREB1L in BRCA and its effect on MDA-MB-231 cells migration and invasion abilities. A Expression of GREB1L in BRCA tissues and paired adjacent tissues via immunohistochemistry (original magnification: 100 ×). (B) The protein level in mammary epithelial cells and BRCA cells. C The mRNA level in mammary epithelial cells and BRCA cells. D, E Stable knockdown of GREB1L in MDA-MB-231 cells. Western blotting (D) and qPCR (E) were used to verify the knockdown effect of GREB1L. F, G The effect of GREB1L on the migration (F) and invasion (G) abilities of MDA-MB-231 cells (original magnification: 200 ×). H, I The effect of GREB1L on the proliferation (H) and colony formation (I) abilities of MDA-MB-231 cells. J Images of the subcutaneous tumors formed in nude mice following injection of stable shCTRL- and shGREB1L-infected MDA-MB-231 cells. K The protein levels of EMT-related genes in the two groups of nude mouse tumors. shCTRL negative control, shGREB1L: shRNA for silencing GREB1L, Data are presented as the mean ± sd. of at least three independent experiments