Fig. 2

piRNA-MW557525 inhibits the proliferation and promoted the apoptosis of NB cells. A piRNA-MW557525 expression was measured to confirm the mimics and inhibitor efficiency in SK-N-SH and SH-SY5Y cells. B CCK-8 assay was performed to determine the proliferation of NB cells transfected with piRNA-MW557525 mimics, inhibitor and NC. C Flow cytometry analysis was performed to determine the apoptosis after piRNA-MW557525 mimic, inhibitor or NC transfection of SK-N-SH and SH-SY5Y cells. D The relative expression of PCNA, Bcl-2, Bax and cleaved caspase-3/8/9 was detected by western blot analysis. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. vs. piRNA-NC