Table 2 Clinical translation and practical considerations of single-cell RNA sequencing in neurological and neurosurgical research

Translational potential of scRNA-seq [68, 69, 71]

ScRNA-seq enables the identification of biomarkers for neurosurgical diseases

Facilitates precision medicine and personalised therapies

Contributes to therapeutic discovery

Considerations for clinical implementation [70, 74, 75]

Standardisation of protocols, quality control, and analysis pipelines

Importance of sample acquisition and preservation

Scalability and cost-effectiveness

Data interpretation and integration

Clinical trials and therapeutic validation [76]

scRNA-seq aids in treatment response assessment and identification of predictive biomarkers

Monitoring disease progression during clinical trials