Fig. 5

Proteomic changes of esophageal squamous carcinoma cells (ESCC) KSYE30 cells treated with esketamine. A Schematic workflow for the analysis of differential expressed proteins (DEPs) via mass spectrometry. B PCA analysis checked the grouping situation of nine samples from three different groups. The heatmap (C), volcano map (D), GO analysis (E) and KEGG analysis (F) for DEPs between KYSE30 treated with 0 or 1 Mm esketamine. The heatmap (G), volcano map (H), GO analysis (I) and KEGG analysis (J) for DEPs between KYSE30 treated with 0 or 2 mM esketamine. K Heat map for DEPs in differential groups of KSYE30 cells treated with increasing dose of esketamine (0, 1, 2 mM). Venn diagram (L) showed the common DEPs between 0 vs 1 mM ketamine group and 0 vs 2 mM ketamine group. GO analysis (M) and KEGG analysis (N) for these common DEPs