Fig. 2

YTHDF1 knockdown decreased the AR level, viability and m6A methylation level of LNCAP cells in vitro. A mRNA expression of YTHDF1 in LNCAP cells after transfected with si-YTHDF1-1/2/3. ** P < 0.01, compared to siNC group. B Protein expression of YTHDF1 after transfected with si-YTHDF1-1 after cultured for 24 h, 48 h, and 72 h. *P < 0.05, compared to 0 h; ^#P < 0.05, compared to 24 h. group. C AR mRN expression in LNCAP cells after YTHDF1 knockdown. D AR concentration in LNCAP cells after YTHDF1 knockdown by enzyme-linked immunosorbent assay. E Viability of LNCAP cells after YTHDF1 knockdown with different culture time detected by cell counting kit-8. F m6A methylation level after YTHDF1 knockdown detected by a RNA Methylation Quantification Kit. *P < 0.05, ** P < 0.01, compared to siNC group