Fig. 3

m6A-sequencing identified TRIM68 as a direct target of YTHDF1. A Expression levels of TIPARP, TRIM68 and SMARCA4 after YTHDF1 knockdown were detected by qRT-PCR. B. Expression level of TRIM68 after YTHDF1 knockdown was detected by western blot. A, B Expression of TRIM68 after transfected with siRNA and TRIM68-overexpressed plasmid was detected. Cell viability (E), apoptosis (F), wound healing (G), and invasion (H) in si-YTHDF1, si-TRIM68 and si-YTHDF1 + TRIM68-overexpression (OE) groups were detected by CCK-8, flow cytometry, wound healing and transwell assays, respectively. Scale bar = 50 μm. *P < 0.05, ** P < 0.01, compared to siNC group;^#P < 0.05, ^## P < 0.01, compared to YTHDF1 siRNA group; ^&P < 0.05, ^&& P < 0.01, compared to TRIM68 siRNA group