Fig. 4

TRIM68 reversed the effects of YTHDF1 knockdown in PC in vitro. A m6A methylation level in PC cells of si-YTHDF1, si-TRIM68 and si-TRIM68 + OE-TRIM68 groups. B, C mRNA and protein levels of YTHDF1, TRIM68 and AR in PC cells of si-YTHDF1, si-TRIM68 and si-TRIM68 + OE-TRIM68 were detected by qRT-PCR and western blot. D Level of AR in cell supernatant in si-YTHDF1, si-TRIM68 and si-TRIM68 + OE-TRIM68 groups was detected by ELISA. *P < 0.05, **P < 0.01, compared to siNC group; ^#P < 0.05, ^##P < 0.01, compared to YTHDF1 siRNA group; ^&P < 0.05, ^&&P < 0.01, compared to TRIM68 siRNA group