Fig. 5
PPARD is raised in osteosarcoma and inhibits osteosarcoma cell proliferation and transfer. A Cellular lysates from osteoblasts and different osteosarcoma cell lines were collected for immunoblotting. The PPARD expression is quantified by ImageJ software in the corresponding column at right. B The expression level of PPARD in MG-63 and Saos-2 cells after knockdown. C Detection of proliferation rate of MG-63 and Saos-2 cells by CCK-8 after PPARD knockdown. D The ability of colony formation in MG-63 and Saos-2 cells after PPARD knockdown. Cell numbers are quantified in the corresponding column at right. E, F The wound healing showing the migration of MG-63 and Saos-2 cells after knockdown PPARD, scale bar = 200 μm. The results are quantified in the corresponding column at right