Fig. 1

Identification of neural cells differentiated from AD-iPS cells. The iPS cells derived from peripheral blood monocytes of AD patients (AD-iPS) or normal healthy control (Normal-iPS) were treated with retinoic acid and cultured in a 3D human neural cell culture system to induce neural differentiation. A–C Immunofluorescence staining was performed to access the expression of neural marker protein Nestin on days 7, 21 and 35 of differentiation, respectively. C–F Western blot assay was used to detect the protein expression of AD pathological marker proteins including Aβ42, p-Tau and APP on days 7, 21 and 35 of differentiation. N = 6. Data from at least three independent experiments were presented as mean ± SEM. **P < 0.01