Fig. 2

TFR1 was upregulated during AD-iPS cell differentiation. A, B Western blot assay was performed to measure on days 7, 21 and 35 of differentiation, respectively. C Intracellular total iron was evaluated using atomic absorption spectrometer. D ROS level was detected using commercial ROS detection kits on days 7, 21 and 35 of differentiation, respectively. A, E Western blot assay and F, G immunofluorescence staining were utilized to access TFR1 protein level on days 7, 21 and 35 of differentiation, respectively. H, I The levels of TFR1 in frontal cortex tissues of AD patients and normal controls were detected using Western blot analysis. N = 6. Data from at least three independent experiments were presented as mean ± SEM. *P < 0.05, **P < 0.01