Fig. 5

TFR1 interacted with GSK3B and promoted GSK3B expression. A The potential interacting proteins of TFR1 were predicted by using the GeneMANIA website (https://genemania.org/). B The interaction between TFR1 and GSK3B in AD-iPS cell-differentiated neural cells was verified by using Co-IP assay. C, D Cells were transfected with pcDNA-TFR1, sh-TFR1, and their negative controls, respectively. GSK3B protein level was accessed with Western blot assay. E, F Western blot assay and G, H immunofluorescence staining was employed to access GSK3B protein level on days 7, 21 and 35 of differentiation, respectively. N = 6. Data from at least three independent experiments were presented as mean ± SEM. **P < 0.01