Fig. 6

PDT induces antitumor effects and enhances PD-L1 and HIF-1α expression in vivo. A Schematic illustration of the experiment design to examine PDT responses. B Changes in tumor volume with time after PDT. C Tumor weight was tested after being stripped from mice. D HIF-1α, PD-L1, Bax and Bcl-2 protein expression by western blot assay in A549 tumors after PDT. E TUNEL positive cells in PDT and control group. F Relative expression of HIF-1α, PD-L1, Bax, Bcl-2 mRNA levels by real-time PCR in A549 tumors after PDT. G Correlation analysis among all the tissue samples of HIF-1α and PD-L1 mRNA expressions, and Bax/Bcl-2 and HIF-1α mRNA expressions. ^* p < 0.05, ^** p < 0.01, ^***P < 0.001, ^*.^***p < 0.0001