Fig. 2

Circulating and tissue levels of COMP and ADAMTS7 among different time points during rabbit models. ELISA assays measuring. A sCOMP and B sADAMTS7 at 0 week (before surgery), 1 week, 2 weeks and 4 weeks after acupuncture. One-way repeated measures ANOVA was conducted to compare the concentration of sCOMP and sADAMTS7 among the different patient groupings, and Bonferroni post-hoc tests were used to pairwise comparisons, ^* indicating P ≤ 0.05 compared to 0w group; ^** indicating P ≤ 0.01 compared to 0w group. C Western blotting analyses of the expression of COMP in rabbit IVD tissues in different time points. IHC stainings of COMP (D) and ADAMTS7 (E) in rabbit IVD tissues. F The expression level of COMP and ADAMTS7 were quantified as positive rate, results were shown as mean ± SD. P value was calculated with t test. ^** indicating P ≤ 0.01 compared to CTR. G RT-qPCR analysis of COMP and ADAMTS7 expression in rabbit IVD tissues at different time points. Scale bar, 50 µm