Fig. 13

PIAS3 promotes metastasis of HCC-LM3 and MHCC97-H cells. A The mRNA expression of PIAS3 in human liver cancer tissue and normal tissue. B The protein level of PIAS3 in human liver cancer tissue and normal tissue. C Based on the HPA database, representative immunohistochemical staining of PIAS3 in normal and tumor tissues of LIHC. D The expression of PIAS3 protein in human hepatocarcinoma and para-cancerous tissues was analyzed by immunohistochemistry. E The mRNA expression of PIAS3 in HCC-LM3 and MHCC97-H cells. F PIAS3 protein expression in HCC-LM3 and MHCC97-H cells. G The wound healing assay suggested that PIAS3 overexpression promoted cell migration of HCC-LM3 and MHCC97-H cells. H, I Quantitative analysis of wound healing percentage in HCC-LM3 and MHCC97-H cells. J The effects of PIAS3 on cell migration and invasion were examined by transwell assays in HCC-LM3 and MHCC97-H cells. (K-N) Quantitative analysis of cell migration and invasion in HCC-LM3 cells and MHCC97-H cells. *p < 0.05;**p < 0.01;***p < 0.001