Fig. 11

Experimental validation of characteristic gene expression in colorectal cancer. A Detection of KCTD9 mRNA expression patterns in CRC and paracancerous tissue by RT-qPCR. B, C Detection of KCTD9 protein expression patterns in CRC tissues and paracancerous tissue samples by the western blot (B) and the immunohistochemistry (C) in the tissue microarray. IOD Integral optical density, I–IV TNM staging of patients, alive/dead survival information of the patient in the 5th year after surgery, KCTD9 potassium channel tetramerization domain containing 9, CRC colorectal cancer, RT-qPCR real-time quantitative polymerase chain reaction