Fig. 7

Knockdown FNDC3B expression hampers proliferation and metastasis ability of PC cells. A FNDC3B expression in six PC cell lines and one normal pancreatic epithelial cell line via western blot analyses. B The efficacies of FNDC3B siRNAs were confirmed via western blot analyses. C CCK-8 and D colony formation assays demonstrated that cell proliferation ability impaired when FNDC3B expression knockdown. E Wound healing and F transwell migration and invasion assays showed that cell metastasis ability attenuated when FNDC3B expression knockdown. G Protein levels of EMT and cell cycle related regulators were evaluated by western blot analyses. H Knockdown of FNDC3B decreased the tumor growth in orthotopic xenograft models established using PANC-1 cells. Representative IVIS images of nude mice are shown. *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001. FNDC3B fibronectin type III domain containing 3B, ns not significant, PC pancreatic cancer