Fig. 7

SCFAs treatment alleviated LPS/nigericin-induced inflammation in human THP-1 cells in vitro. A–C Western blotting showed SCFAs treatment significantly elevated GPR43 expression and decreased NLRP3 expression in LPS/nigericin-induced human THP-1 cells. β-Tubulin and GAPDH were utilized as endogenous control. A.U., arbitrary units; n = 4 for each group; T-test. D The levels of IL-1β in the culture supernatant of human THP-1 cells stimulated with or without SCFAs, LPS, and nigericin in vitro. T-test; n = 3 for each group. *P < 0.05; **P < 0.01; ***P < 0.001; data are mean ± SEM