- Open Access
Reflex influence of carotid baroreceptor inactivation on respiratory resistance in humans
European Journal of Medical Research volume 14, Article number: 126 (2009)
Our previous study demonstrated that selective carotid baroreceptors activation decreases airway resistance. The aim of the present study was to evaluate the effect of carotid baroreceptor inactivation on the reflex change of respiratory resistance. Twenty healthy men aged between 20 and 25 were included in the study. Selective inactivation of carotid baroreceptors was induced by generating a positive pressure of 40 mmHg for 5 s in two capsules placed bilaterally on the neck over the bifurcation of the carotid arteries. The oscillatory method (Siregnost FD5, Siemens) was used to measure continuously respiratory resistance. Inactivation of carotid baroreceptors produced a short increase in respiratory resistance by 0.39 ± 0.01(SE) mbar/l/s, i.e., 21.7% above the resting level. We conclude that in humans, carotid baroreceptors might have a background contribution to bronchodilator tone. This observation seems to be important for clinical situations of impairment of baroreflex function.
The main baroreceptive areas are in the carotid sinuses and these are the regions which have been most studied in humans. Various non-specific stimuli can be used to examine reflex control. The use of an airtight chamber applied to the subject's neck allows application of either a negative pressure which increases carotid transmural pressure and activates of the baroreceptors, or a positive pressure which has the opposite effect and inactivates the receptors . It is plausible that this stimulus (positive or negative) alters the firing of other mechanoreceptors located in the neck region beside the carotid baroreceptors, which includes the upper airway receptors in the trachea and larynx. A new more selective method of activation and inactivation of carotid baroreceptors in humans has been described ; namely, instead of one neck chamber, two separate capsules placed on the surface of the skin over the carotid bifurcations. The use of two separate capsules (chambers) makes it possible to apply stimuli to the left and right carotid sinus, without activation or inactivation of the other neck's mechanoreceptors. It is known from studies in animals that the reflex effects of activating the carotid sinus baroreceptors involve breathing and bronchomotor tone [3–5]. Our previous study in man has demonstrated that carotid baroreflex activation is not confined to the cardiovascular system (produces bradycardia), but also decreases airway resistance. The aim of the present study was to evaluate the opposite, i.e., the influence of carotid baroreceptors inactivation on respiratory resistance in humans.
Materials and methods
Twenty healthy male volunteers aged between 20 and 25 were included in the study. The study protocol was approved by a local Ethics Committee and the subjects gave informed consent to study procedures. All experiments were performed at rest, in the seated recumbent position.
Inactivation of Carotid Baroreceptors
In order to inactivate carotid baroreceptors a two-neck-chamber method was applied. The selective inactivation of carotid baroreceptors was induced by generating a positive pressure of 40 mmHg for 5 s in two capsules placed bilaterally on the neck over the bifurcation of the carotid arteries. The stimuli were applied 250 ms after the R-wave during the expiratory phase of breathing. The maximal rate of air pressure increase was 300 mmHg per second.
The following variables were recorded continuously:
R-R intervals, measured from ECG;
Respiratory phase and the rate of breathing by means of a termistor placed at a nose orifice;
Air pressure in the neck chambers;
An average R-R interval was calculated from a 10-s recording preceding the onset of stimulus and is referred to as the baseline. The cardiac baroreflex response was expressed as a gain of heart response (GHR) and calculated as the peak value of the R-R shortening during the 5 s after the onset of stimulus, compared with the baseline level, and divided by the neck pressure change.
Measurement of Respiratory Resistance
The oscillatory method (Siregnost FD5, Siemens, Germany) was used to measure continuously respiratory resistance, as described in detail elsewhere .
We took the calculated GHR as the expression of a cardiac response to carotid baroreceptors inactivation. The mean GHR was -4.013 ± 0.030(SE) ms/mmHg. Inactivation of carotid baroreceptors produced a short increase in respiratory resistance by 0.39 ± 0.01(SE) mbar/l/s, i.e., 21.7% above the resting level.
Although the use of neck chambers seems to provide a discrete baroreceptors stimulus, there are some limitations and considerations to take into account. The immediate response of pulse interval is likely to be due mainly to baroreceptor inactivation. There is evidence that about 86% of the applied positive pressure is transmitted to the deep structures of the neck . However, this response rapidly becomes smaller due presumably to some adaptation of the receptors but also to 'buffering' by the resulting response which exerts an effect on other baroreceptors . This consideration limits the use of this technique in the study of vasomotor responses. These, being sympathetically mediated, occur more slowly than vagally mediated effects on the heart . In the present study, only the heart response to baroreceptor inactivation, calculated as a gain in the heart response, was measured. The maximum shortening of the pulse interval observed during the 5 s of stimulus action seems to be vagally mediated (inhibition of vagal activity) with a small component of sympathetic activation and may be considered as a relatively pure cardiac response to carotid baroreceptors inactivation.
The main finding of this study is that, in humans, carotid baroreceptors inactivation evoked reciprocal effects on vagal cholinergic control of the heart and airways: a decrease in cardioinhibitory tone and an increase in bronchoconstrictor tone. Our previous study showed a contrary effect of activation of carotid baroreceptors: an increase in cardioinhibitory tone and a decrease in bronchoconstrictor tone . The motor neurons of the vagus nerve are situated in two nuclei: the dorsal vagal nucleus and the nucleus ambiguus, with the parasympathetic motor neurons innervating the sinus node and airway in the latter nucleus . Cardioinhibitory cells tend to be more caudal and ventral than the presumed bronchoconstrictor neurons . Thus, morphological and functional evidence is consistent with there being two separate vagal efferent pathways of the carotid baroreflex. Inactivation of the carotid baroreflex in the present study evoked both synergic reflex responses: circulatory response - increase in heart rate, and ventilatory response - increase in respiratory resistance, in effect resulting in increases in inspiratory effort and in venous return.
Numerous studies have shown that respiratory depression due to increased arterial blood pressure preferentially affects upper airway motor output in humans [11, 12]. Because increases in blood pressure increase the severity of upper airway obstruction by increasing pharyngeal collapsibility , "baroreceptors have been implicated in the regulation of upper airway patency" .
We conclude that in humans carotid baroreflex decreases respiratory resistance. This tonic bronchodilator influence, resulting in a decrease in inspiratory effort, may uphold the imbalance between the activities of the upper airway and diaphragm and chest wall inspiratory muscle groups. This observation seems noteworthy for clinical situations of impairment of baroreflex function.
Conflicts of interest
The authors declare that they have no competing interests.
Ludbrook J, Mancia G, Ferrari A, Zanchetti A: The variablepressure neck chamber method for studying the carotid baroreflex in man. Clin Sci Mol Med 1977, 53: 165–171.
Tafil-Klawe M, Raschke F, Hildebrandt G: Functional asymmetry in carotid sinus cardiac reflexes in humans. Eur J Appl Physiol 1990, 60: 402–406. 10.1007/BF00713507
Brunner MJ, Sussman MS, Greene AS: Carotid sinus baroreceptor reflex control of respiration. Circ Res 1982, 51: 624–636. 10.1161/01.RES.51.5.624
Nadel JA, Widdicobme JG: Effect of changes in blood gas tension and carotid sinus pressure on tracheal volume and total lung resistance to airflow. J Physiol 1962, 163: 13–33.
Schultz HD, Pisarri TD, Coleridge HM, Coleridge JCG: Carotid sinus baroreceptors modulate tracheal smooth muscle tension in dogs. Circ Res 1987, 60: 337–345. 10.1161/01.RES.60.3.337
Von Korn V: Die Bestimmung des Atemwiderstandes mit dem oszillatorischen Messprinzip, Siregnost FD5. Dustri Verlag 1979, 16–40.
Mancia G, Ferrari A, Gregorini L, Valentini L, Ludbrook J, Zanchett A: Circulatory reflexes from carotid and extracarotid baroreceptor areas in man. Circulation Res 1977, 41: 309–315. 10.1161/01.RES.41.3.309
Mary D, Hainsworth R: Methods for the study of cardiovascular reflexes. In Cardiovascular reflex control in health and disease. Edited by: Hainsworth R, Mark AL. W.B. Saunders Company Ltd; 1993:1–34.
Mc Allen RM, Spyer KM: Two types of vagal preganglionic motoneurones projecting to the heart and lungs. J Physiol 1978, 282: 353–364.
Kalia M: Brain stem localization of vagal preganglionic neurons. J Aut Ner Syst 1981, 3: 451–481. 10.1016/0165-1838(81)90081-3
Garpestad E, Basner RC, Ringeler J, Lilly J, Schwarzstein R, Weineberger SE, Weiss JW: Phenylephrine-induced hypertension acutely decreases genioglossus EMG activity in awake humans. J Appl Physiol 1992, 72: 110–115. 10.1063/1.352167
Wasicko MJ, Knuth SL, Leiter JC: Response of genioglossus EMG to passive tilt in man. J Appl Physiol 1993, 74: 73–81.
Mayor AH, Schwartz AR, Rowley JA, Willey SJ, Gillespies MB, Smith PL, Robotham JL: Effect of blood pressure changes on air flow dynamics in the upper airway in the decerebrate cat. Anesthesiology 1996, 84: 128–134. 10.1097/00000542-199601000-00015
Stella MH, Knuth SL, Barlett D Jr: Respiratory response to baroreceptor stimulation and spontaneous contractions of the urinary bladder. Res Physiol 2001, 124: 169–178. 10.1016/S0034-5687(00)00215-2
About this article
Cite this article
Klawe, J., Tafil-Klawe, M., Lewandowski, A. et al. Reflex influence of carotid baroreceptor inactivation on respiratory resistance in humans. Eur J Med Res 14, 126 (2009). https://doi.org/10.1186/2047-783X-14-S4-126
- airway resistance
- carotid baroreflex