- Case report
- Open Access
An unsuspected ameloblastoma in the subpontic region of the mandible with consideration of pathogenesis from the radiographic course
European Journal of Medical Researchvolume 15, Article number: 135 (2010)
The purpose of this report is to document a case of unsuspected ameloblastoma involving the right man dibular subpontic region in a 38-year-old Cambodian female patient. This lesion was purportedly preceded by multiple radiolucencies which were diagnosed as radi cular cysts and treated a few times in the past years by enucleation followed by endodontic therapy of the affected teeth. Bridgework restoration of the partially edentulous area was performed. This case report de monstrates radiographic changes that occurred in the periods before and after the diagnosis of amelo blas to ma. The case may represent an example of radicular cysts and ameloblastoma occurring as a collision phenomenon, or the ameloblastoma may have arisen as a result of neoplastic transformation of the lining epi thelium in an inflammatory odontogenic epithelial cyst.
The World Health Organization defined ameloblastomas as a slowly-growing, locally-invasive odontogenic epithelial neoplasm . It is the second most common odontogenic tumor occurring without gender predilection and affecting a wide age range. The clinical, radiological and histological features of this neoplasm have been well-characterized [1, 2]. The general consensus is that the ameloblastoma originates in the enamel organ and its derivatives as well as in the epithelial lining of developmental odontogenic cysts [1, 3]. Reports of ameloblastoma arising as a consequence of neoplastic transformation of the lining epithelium in an inflammatory odontogenic cyst are virtually unknown.
In this report, we present a case of ameloblatoma, which may represent an example of radicular cysts and ameloblastoma occurring as a collision phenomenon, or perhaps the ameloblastoma arose as a result of neoplastic transformation of the lining epithelium in an inflammatory odontogenic epithelial cyst.
History of present complaint
In January 2007, a 38-year-old Cambodian female patient presented to the Department of Oral Surgery, Faculty of Dentistry, University of Health Sciences, Phnom Penh, Cambodia, with a complaint of painful swelling with pus discharge from her right lower jaw for the past one month.
Past dental history
The patient gave a history of removal of cysts from the same site a year earlier. According to the dentist attending to the patient, cystic lesions which looked clinically like radicular cysts were removed a few times. Based on the histopathology report of the specimens, the diagnosis was 'an inflammatory odontogenic cyst, most probably a periapical cyst (radicular cyst)'. However, these lesions kept recurring at the same site but seemed to have reduced in size following the last surgery. During the same period, the patient underwent endodontic treatment of her left mandibular central incisors, right mandibular central and lateral incisor, and right mandibular first premolar. Ceramic restorations of her right mandibular lateral incisor, right mandibular first premolar and right mandibular second molar as well, as bridgework replacing missing right mandibular canine and right mandibular second premolar and first molar, were also constructed. Her most recent history was of a painful swelling over the same area one month earlier, which improved after a course of antibiotics.
Intraoral examination revealed a fluctuant swelling with buccal bony expansion over the right mandibular premolar-molar region and extending anteriorly along the labial alveolus. A discharging sinus was seen in the lingual mucosa between the right and left mandibular central incisors.
Orthopantomogram taken in 2006 showed a well-defined unilocular radiolucency located in the partially-edentulous body of the man di ble, i.e. between the right mandibular first premolar and second molar (Figure 1A). The radiolucency had mildly scalloped, sclerotic margins, and fine bony septa traversing the center. Root resorption of the right man dibular first premolar and second molar was evident.
The orthopantomogram taken in January 2007 showed considerable bone filling-in of the original radiolucency between the right mandibular first premolar and second molar (Figure 1B). However, a new unilocular radiolucency appeared to have formed between the right mandibular lateral incisor and right mandibular first premolar. This new radiolucency extended anteriorly to the apices of the left and right mandibular central incisors. Radiographically it appeared to be separated from the original radiolucency by bony septa.
Under local anesthesia, the new radiolucent lesion was enucleated and submitted for histopathological examination. A diagnosis of 'cystic ameloblastoma' was made.
Follow-up radiographic examination
An orthopantomogram taken in November 2007 showed considerable bone-filling of the previously enucleated site, i.e. between the right mandibular lateral incisor and first premolar (Figure 1C). However, a recurrent radiolucency had emerged between the right mandibular first premolar and second molar extraction socket. The superstructure spanning the right mandibular second premolar and first molar had been removed.
The orthopantomogram taken in June 2008 showed that the radiolucency between the right mandibular first premolar and second molar extraction socket had shrunk in size (Figure 1D). The socket outline of the right man di bular second molar was still visible. There was no evidence of recurrence in the right mandibular canine region.
In August 2008, surgical excision of the lesion from the mesial side of the right mandibular canine to the third molar was performed. The histopathology report stated that the lesion was a 'follicular ameloblastoma'. However, no further information regarding the outcome of this treatment was available as the patient was lost to follow-up.
Histopathologically, the soft tissue specimen enucleated from the right mandibular canine region in 2007 showed cystic features (Figure 2A). The cystic wall mainly consisted of a dense fibrous connective tissue wall. In the cyst wall, there were some inflammatory cell infiltration especially in the inner layer. The lining epithelium, in parts, was some cell-layered cuboidal and/or squamous in shape (Figure 2B). In most parts of the cyst wall, the lining epithelium showed odontogenic in shape, resulting ameloblastomatous proliferation. In particular, the most peripheral layer of cells was columnar with hyperchromatic nuclei, and lined up in a palisaded fashion. Furthermore, there were small ameloblastoma islands in the connective cyst wall, suggesting mural ameloblastoma (Figure 2C, 2D). The intraluminar cells were sometimes loosely arranged. Furthermore, extensive proliferation occurred into the cystic spaces (Figure 2E). In the connective tissues which formed the cyst wall, there were budding features with occasional formation of ameloblastoma nests (Figure 2F).
Ameloblastoma is classified as a benign, locally-infiltrative odontogenic neoplasm, which is composed of proliferating odontogenic epithelial nests within a fibrous stromal tissue. Some variants have been subclassified as follows: solid/multicystic, extra osseous/peripheral, desmoplastic, and unicystic . Odontogenesis is a complex biological process, and this process is directly reflected in the development of odontogenic neoplasms, especially ameloblastomas. It is thought that the above-mentioned variants are due to the developmental complex system [4, 5].
Intraosseous small ameloblastomas that sometimes appear within the jawbone are not well-studied. The lesions are not clinically detected; therefore, clinical-oriented evidence is limited at present . In the literature, there has been some discussion on the cellular sources of unsuspected small ameloblastoma arising in the jawbone. Furthermore, the oriented histogenesis is sometime discussed. Houston et al. (2007)  reported a case of ameloblastoma arising from dentigerous cyst With regards to the neoplastic or atypical proliferative change from the lining epithelium of an odontogenic cyst, Antoh et al. (1993)  described such an example in a case of radicular cyst. Careful follow-up is necessary after treatment, since some cysts have the possibility of neoplastic transformation.
As mentioned previously, unsuspected ameloblas tomas are usually small, asymptomatic and confined to the alveolar bone [6, 8]. Their radiographic appearance is usually that of a nondescript lytic lesion. None of these features were observed in the current case. Instead, the case presented as an expansive fluctuant swelling associated with pain and a discharging sinus when the patient attended the Oral Surgery Clinic in January 2007. Radiographically, a unilocular lesion straddling the bone area between the right mandibular lateral incisor and first premolar was observed. Ameloblastoma remained unsuspected at this stage possibly because the clinical and radiological findings were nonspecific and could fit in a variety of inflammatory, neoplastic or cystic conditions of the jawbone.
With regards to the current case, the precise relationship between the radicular cysts enucleated from the right mandibular premolar-molar region in 2006, ameloblastoma diagnosed in the right mandibular canine region in January 2007, and follicular ameloblastoma in the right premolar-molar region in August 2008 remains an enigma. It is likely that the ameloblastoma located in the right mandibular canine and premolar-molar regions are one and the same lesion. Although plain radiographs showed a bony septa separating the two lesions, cancellous spread not detectable on plain radiographs may have occurred. The link, if any, with the radicular cysts that preceded the diagnosis of ameloblastoma in the right mandibular premolar-molar region is also unclear. Evaluation of the past dental history of this case showed that the first lesion diagnosed histopathologically was an apical inflammatory odontogenic cyst (radicular cyst) and this was removed a few times. In January 2007, the lesion enucleated from the right mandibular canine region was histopathologically examined and diagnosed as a cystic ameloblastoma. In consideration of the close proximity of the ameloblastoma in the site where the radicular cysts were previously enucleated. We therefore speculated that this case possibly represented an example of ameloblastoma arising from a cyst. We theorized that the cyst lining epithelium progressed and underwent ameloblastomatous change. Histopathologically this is a feasible concept because the epithelial cell rests of Malassez, which gives rise to radicular cyst, have been implicated in the origin of multiple odontogenic cysts and neoplasms . In addition, the histopathological examination results are consistent with the above consideration. Furthermore, we observed small follicular ameloblastoma islands in the cyst wall connective tissues. The final histopathological diagnosis was follicular ameloblastoma. Another plausible explanation is that these entities may represent a collision phenomenon. The occurrence of collision lesions, including those of an odontogenic epithelial nature, is not uncommon in the jawbones [9–13]. That only radicular cysts were enucleated and diagnosed in 2006 may be due to the fact the ameloblastoma developing in the same area was in its incipient stage and therefore undetected.
In summary, this case reported here may represent an example of radicular cyst and ameloblastoma occurring as a collision phenomenon, or of an amelo blastoma arising from a result of neoplastic transformation of the lining epithelium in an inflammatory odontogenic (radicular) cyst. Although, their true relationships remain unknown, nonetheless the considered pathogenesis was consistent with the course of histopathology and radiography.
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This research was supported in part by a Grant-in Aid for Scientific Research (C) (20592349) from the Japan Society for the Promotion of Science.