Study of T Cell subsets and IL-7 protein expression in HIV-1-infected patients after 7 years HAART
© I. Holzapfel Publishers 2011
Received: 23 May 2011
Accepted: 10 November 2011
Published: 21 December 2011
To study the changes in T cell subsets and IL-7 in HIV-1-infected patients after seven years of highly active antiretroviral therapy (HAART).
Seventy-five individuals were included in this study (25 with effective HAART, 18 with ineffective HAART, 17 untreated HIV+ patients, and 15 volunteers in the HIV negative control group). The counts of CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells as well as the IL-7 protein expression was measured at 5 time points during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. The expression of CD127 on CD3+ T cells was measured by flow cytometry at a single time point (after 7 years) in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group.
At baseline CD4+ T cell counts of HIV-1-infected patients were lower than that in the control group (p < 0.01), whereas the CD8+, CD8/HLADR+ and CD8/CD38+ T cell counts were higher than those in the control group (p < 0.01). After seven years of effective HAART, the CD4+ T cell counts had increased and the CD8+ T cell count had decreased, although not to the normal levels (p < 0.05). Both the CD8/HLADR+ and CD8/CD38+ T cell counts had gradually approached those of the control group (p > 0.05). In the ineffective HAART group, the CD8/CD38+ T cell count had not decreased significantly, and CD8/HLADR+ T cell count gradually decreased. Before treatment, IL-7 serum levels of patients were significantly higher than that in the control group (p < 0.01). After seven years of effective HAART, IL-7 levels had gradually decreased, but were still higher than in the control group (p < 0.01). The CD127 expression on CD3+ CD8+ T cells in effective HAART patients was higher than in untreated HIV+ patients (p < 0.05), but was lower than that in the control group (p < 0.05). CD127 expression on CD3+ CD4+ T cells was not significantly different among the control group, untreated HIV+ patients and effective HAART group.
After seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 in HIV-1-infected patients had been partially restored, and the abnormal immune activation has significantly diminished.
Human immunodeficiency virus-1 (HIV-1) mainly attacks CD4+ T cells of the immune system, which leads to a serious impairment of immune function. The pathological changes associated with HIV-1 infection include changes in both the population and function of CD4+ T cells, as well as abnormal activation of the immune system . Highly active antiretroviral therapy (HAART) can inhibit virus replication in vivo, and thereby inhibit the progression of AIDS . It has been reported that HAART can partially restore immune function in HIV-1-infected patients; however, how long the recovery period is and how long the inhibition of abnormal immune activation would last remain unknown. Moreover, there is still a lack of studies on the function of long-term HAART on immune restauration in HIV-1-infected patients.
IL-7, which plays a crucial role in T cell homeostasis, also regulates peripheral naive T cell survival and rhIL-7 was found to simultaneously increase CD4+ and CD8+ T cell counts, particularly memory T cells. Furthermore, the expression level and regulatory mechanism of the IL-7Rα chain (CD127) is positively correlated with the lifetime of T cells in peripheral blood. The expression of CD127, which is a specific surface marker of T cells, can be lowered by HIV-1-specific lymphocytes, thereby impairing its regulatory function. Low CD127 levels on CD8+ T cells lead to a proliferation of HIV (see also discussion).
In this study, the changes in T lymphocyte subsets and peripheral blood IL-7 protein expression was investigated during a period of seven years in patients starting HAART (baseline) and in the HIV negative control group. In addition, the expression of CD127 on CD3+ T cells were measured by flow cytometry in patients with HAART and was compared with untreated HIV+ patients and the HIV negative control group. By analysing differences among those groups, we aimed to investigate the immune restauration achieved by seven years of HAART in HIV-1-infected patients.
Materials and methods
The HIV-1-infected adults were retrospectively reviewed between 1998 and 2009 at the St. Josef Hospital, Ruhr-University bochum, Germany and Qing Chun Hospital, Zhejiang Province, China. All the patients infected by HIV-1 had no severe opportunistic infections. Patients receiving more than seven years of HAART were divided into two groups: Effective group (group A): plasma HIV-RNA lower than the level of detection (50 copies/mL) after 1 year of HAART. Ineffective group (group B): plasma HIV-RNA higher than the level of detection (50 copies/mL) after 1 year of HAART. The cause of ineffective therapy (e.g resistance or lack of compliance) was not investigated. The plasma HIV-RNA level of the patients was documented annually. If the plasma HIV-RNA level was repeatedly above detection level antretroviral therapy was changed within one year, but was still not stable after 7 years. Some analyses were compared with an untreated group (group C): HIV-1 infected individuals who didn't receive antiretroviral therapy. As a control group served HIV negative volunteers with no history of acute or chronic illnesses, especially immunologic diseases and tumours, no infections during the previous two months and no abnormal findings in physical examinations.
Sample collection and processing
Peripheral blood (7 mL) was taken from each of the subjects, and the samples were drawn into tubes with EDTA. Half of the sample was measured by flow cytometry (Becton, Dickinson and Company). Plasma extracted from the other half of the sample was used to measure the viral load and IL-7 protein expression. The blood samples of the patients who received long-term HAART were taken annually.
Detection of plasma HIV-RNA
An HIV-1 Monitor 1.5 commercial kit (Roche) and COBAS AmpliSensor-PCR were used for quantitative analysis of HIV-RNA in the plasma samples. The measurements were performed according to the manu facturers' instructions.
Three different combinations of fluorescent antibodies (CD8+ CD127+ T, CD8+ CD45RO+ CD127+ T and CD8+ CD45RA+ CD127+ T) were separately drawn into three tubes of 50 μL EDTA blood from each sample. Flow cytometry (Becton, Dickinson and Company) and System U software were used to analyse the results. The same method was applied to detect CD4+, CD8+, CD8/CD38+, and CD8/HLADR+ T cells in the peripheral blood. The fluorescent antibodies used were as follows: CD3 FITC, CD4 PerCP 5.5, CD8 APC-H7, CD45RO PECy7, CD45RA PECy7, CD127 PE, CD4 FITC, CD8 FITC, CD38 PE, and anti-HLA-DR APC.
IL-7 Quantitative determination
ELISA was used to determine the concentration of IL7 (μg/L). The absorbance at 450 nm was measured using an ELISA Reader. The reagent was purchased from R & D Company, US. The measurements were performed according to the manufacturer's instructions.
All data was denoted by mean and standard deviation ( ± SD). To compare the T cell subsets during the long-term effective HAART, matching Student's t-test was used. Mann-Whitney U-test was used for analysis of the other continuous data. All data were entered and analyzed using SPSS version 16 packages, and p value of less than 0.05 was considered statistically significant.
The clinical characteristics of the study population
Baseline characteristics of study population
Number of Subjects
47.6 ± 9.1
45.7 ± 10.4
39.7 ± 11.3
40.5 ± 9.7
CD4+ T (cells/[μL) *
≥ 200~ < 350
Changes in CD4+ and CD8+ T cells after seven years of effective HAART
The mean CD4+ T cell count (cells/μL) during HAART
Baseline CD4 (cells/nL)
In addition, before treatment, the CD8+ T cell counts in the above patients were significantly higher than that in the control group (1255 vs 593 cells/μL, p < 0.01). After seven years of effective HAART, the CD8+ T cell counts had decreased significantly, but were still higher than that in the control group (911 vs 593 cells/μL, p < 0.05) (Fig 1).
Analysis of CD8/CD38+ and CD8/HLADR+ T cells after seven years HAART
Analysis of IL-7 protein expression after seven years of effective HAART
Changes in CD127 expression on CD3+ T cells after seven years of effective HAART
HIV-1 can, by infecting CD4+ T cells, induce a progressive decline of lymphocytes, a deficiency in immune function and a variety of opportunistic infections. CD8+ T cells are activated in the initial stage of infection as the main defensive barrier, and the cytolytic T lymphocyte response is soon induced to inhibit viral replication. Some transmembrane molecules are highly expressed during the activation period, indicating the activated state of cells [3, 4], for example, HLA-DR and CD38 molecules are indicators of T cell activation. Normally, these two molecules are seldom expressed simultaneously on CD4+ and CD8+ T cells. After infection, the CD38 expression levels of T cells are three to five times higher than normal, and they correlate to disease progression .
In the present study, the changes in CD4+ and CD8+ T cell counts were investigated during seven years of HAART. The study duration was partly chosen because of external factors (exchange of staff between Germany and China). During this course of therapy, CD4+ T cells increased and CD8+ T cells decreased significantly (Figure 1). After the third year of HAART, CD4+ and CD8+ T cell counts were maintained at stable levels, although those were still far from the levels of the control group. This is in accordance with observational cohort studies where a plateau is reached after 2-8 years depending on the baseline count . It has been demonstrated that baseline CD4 count is a significant prognostic indicator for HAART therapy. Some studies showed that patients with the higher baseline CD4 count at HAART initiation have better chance to restore to normal CD4 count [7–9]. In our study, we have done the correlate analysis between baseline CD4 and the recovery CD4 counts after 1, 3, 5 and 7 years. The mean CD4 recovery was higher with high baseline CD4 count, however, the related coefficient analysis was not very satisfactory (P > 0.05). It may be caused by the small sample size.
Meanwhile, the fact that CD8/CD38+ and CD8/HLADR+ T cell counts in peripheral blood decreased and, finally, fell to within the normal range, demonstrated the decrease of abnormal immune activation. However, no statistically significant reduction in CD8/CD38+ T cell counts was observed in the ineffective HAART patients (Figure 3). Although CD8/ HLADR+ T cell counts gradually decreased, the decrease was less than in the effective HAART group (Figure 4). Thus, we conclude that long-term (at least 7 years) effective HAART could have an effect on immune restauration in HIV-1-infected patients, and that continued detection of CD8/CD38+ T cells is an optimal way to quantify and explicate the immune activation levels in HIV-1-infected patients undergoing HAART.
IL-7 plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis, sustaining thymocyte proliferation and survival. It also regulates peripheral naive T cell survival by inducing the production of the anti-apoptotic molecule Bcl-2 . In a recent study, subcutaneous injection of rhIL-7 was found to simultaneously increase CD4+ and CD8+ T cell counts, particularly memory T cells . IL-7 concentration in the peripheral blood of HIV-1-infected patients was proven to be higher than that in healthy people, and it was considered to accompany a decrease in CD4+T cells when the IL-7 production was induced [10, 12]. On the basis of seven years investigation of patients with effective HAART, the IL-7 in peripheral blood decreased during antiretroviral therapy, but was finally maintained at a stable level far above normal levels. We speculate that HAART can inhibit HIV-1 reproduction, restore T cell subsets and IL-7 receptor protein expression, and lower the negative feedback of IL-7 to CD4+ T as described by Chomont et al. . The abnormal levels of IL-7 may be due to an incomplete recovery of the IL-7/CD127 regulation mechanisms after seven years of HAART. Chomont et al. have also demonstrated that HAART can only partially downregulate the HIV reservoir , whereas IL-7induced mitosis of transitional memory T cells can lead to the persistence of the HIV reservoir .
The expression level and regulatory mechanism of the IL-7Rα chain (CD127) is positively correlated with the lifetime of T cells in peripheral blood . CD127, as a specific surface marker of T cells, can be used to clarify two mechanisms: the transformation of effector T cells to memory T cells and the maintenance of memory T cells . The expression levels of CD127 can be lowered by HIV-1-specific lymphocytes, thereby impairing its regulatory function. MacPherson et al. have found that downregulating the expression level of CD127 on CD8+ T cells leads to a proliferation of HIV . However, the CD127 expression in HIV-1-infected patients could be partially recovered after antiviral therapy. Furthermore, patients with high expression levels of CD127 enhanced IL-7 signals, stimulating the expression of Bcl-2, and regulating the survival and proliferation of T cells .
This study indicated that the expression of CD127 on the surface of CD3+ CD8+ T cells in untreated HIV-1-infected patients was lower than that in the controls. After seven years of HAART, the CD127 on CD3+ CD8+ CD45RA+ T cells had increased to normal levels (Figure 6). We speculate that effector CD4+ and CD8+ T cells were activated due to the residual viral replication. The low CD127 expression on CD8+ T cells could favour the apoptosis of effector CD8+ T cells, which abrogates the transformation of effector CD8+ T cells to memory T cells. Therefore, HIV-1-specific CD8+ T cells could not survive longer. However, long-term HAART can inhibit the replication of HIV-1 and enhanced CD127 expression on the surface of T cells, which could restore the quantity and capacity of T cell subsets. There were no statistically significant differences among the CD127 expressions on the surface of CD3+ CD4+ T cells in the effective HAART group, the untreated group and the control group. Although similar results have already been reported [13, 18], the specific mechanisms are not clear and need further study.
In conclusion, after seven years of effective HAART, the quantity and capacity of T cell subsets and IL-7 of HIV-1-infected patients were partially restored, and the abnormal immune activation was significantly reduced. However, seven years of HAART could not achieve complete immune reconstitution. Continued investigation of CD8/CD38+ T cells may help to monitor immune activation in HIV-1-infected patients during long-term HAART.
This work was supported in part by the National Eleventh Five-year Plan Key Program of Major Infectious Diseases of China (Grant No.2008ZX10001-006, No.2008ZX10001-006).
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