Fig. 2

Treatment with Cerebrolysin induces an increment of melanin production. Photomicrographs of pre-treatment (A–C, G–I) and post-treatment scalp biopsies (D–F, J–L) showing brown-black stain to the melanin granules of melanocytes (white arrows). A A–F slides with H and E staining show the brownish melanin color; B G–L slides with Fontana-Masson histochemical staining show the black stains due to argentic deposition of melanin. It is noted that post-treatment sections show a greater number and intensity signal in the hair bulb, hair shaft, and epidermis. A, D, G, J at 100 × magnification; B, C, E, F, K, L at 400 × magnification; C Densitometric quantification of the melanin signal on Fontana-Masson slides. A significant increase (*shows p < 0.01) in melanin signal in the hair bulb was detected in post-treatment biopsies. n = 3. Experiments were independently performed at least in duplicate