Fig. 2

Cell processing of scRNA-seq dataset GSE86469. A Number of genes per cell (nFeature_RNA) and Number of UMIs per cell (nCount_RNA) for scRNA-seq data. B The top 2000 variable DEGs between cells are marked in red, and the top 10 variable DEGs are labeled. C Identification of 20 principal components (PCs) with significant differences (p < 0.05), where we observe an ‘elbow’ around PC6-8, suggesting that the majority of true signal is captured in the first 8 PCs. D Identification of 6 cell clusters by performing t-distributed stochastic neighbor embedding (t-SNE). E Sample sources of each cluster. F Heatmap displaying the top 40 marker genes in the 6 clusters