Identification and validation of core genes for type 2 diabetes mellitus by integrated analysis of single-cell and bulk RNA-sequencing
European Journal of Medical Research volume 28, Article number: 340 (2023)
The exact mechanisms of type 2 diabetes mellitus (T2DM) remain largely unknown. We intended to authenticate critical genes linked to T2DM progression by tandem single-cell sequencing and general transcriptome sequencing data.
T2DM single-cell RNA-sequencing data were submitted by the Gene Expression Omnibus (GEO) database and ArrayExpress (EBI), from which gene expression matrices were retrieved. The common cell clusters and representative marker genes were ascertained by principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), CellMarker, and FindMarkers in two datasets (GSE86469 and GSE81608). T2DM-related differentially expressed marker genes were defined by intersection analysis of marker genes and GSE86468-differentially expressed genes. Receiver operating characteristic (ROC) curves were utilized to assign representative marker genes with diagnostic values by GSE86468, GSE29226 and external validation GSE29221, and their prospective target compounds were forecasted by PubChem. Besides, the R package clusterProfiler-based functional annotation was designed to unveil the intrinsic mechanisms of the target genes. At last, western blot was used to validate the alternation of CDKN1C and DLK1 expression in primary pancreatic islet cells cultured with or without 30mM glucose.
Three common cell clusters were authenticated in two independent T2DM single-cell sequencing data, covering neurons, epithelial cells, and smooth muscle cells. Functional ensemble analysis disclosed an intimate association of these cell clusters with peptide/insulin secretion and pancreatic development. Pseudo-temporal trajectory analysis indicated that almost all epithelial and smooth muscle cells were of neuron origin. We characterized CDKN1C and DLK1, which were notably upregulated in T2DM samples, with satisfactory availability in recognizing three representative marker genes in non-diabetic and T2DM samples, and they were also robustly interlinked with the clinical characteristics of patients. Western blot also demonstrated that, compared with control group, the expression of CDKN1C and DLK1 were increased in primary pancreatic islet cells cultured with 30 mM glucose for 48 h. Additionally, PubChem projected 11 and 21 potential compounds for CDKN1C and DLK1, respectively.
It is desirable that the emergence of the 2 critical genes indicated (CDKN1C and DLK1) could be catalysts for the investigation of the mechanisms of T2DM progression and the exploitation of innovative therapies.
Diabetes, characterized by hyperglycemia caused by absolute or relative insulin deficiency, is a major contributing factor to atherosclerosis, coronary heart disease and premature death . With a significant increase in the global prevalence of diabetes, the population of diabetics is estimated to 642 million by 2040 , at an estimated annual health care cost of $825 billion , making diabetes and its complications important public health issues. In contrast to T1 diabetes, the major subtype of diabetes is T2 diabetes mellitus (T2DM), also known as non-insulin-dependent diabetes, accounting for more than 90% of diabetics . Although extensive research has been done into the pathogenesis of T2DM, the exact mechanism remains largely unknown. Hence, it is imperative to explore its possible specific mechanism and develop corresponding prevention and treatment strategies.
Pancreatic islet cells are critical for maintaining normal blood glucose levels, as damage to pancreatic islet cells can lead to the development and progression of diabetes. With the rapid development of high throughput second generation RNA sequencing (RNA-Seq), transcriptomic analysis of pancreatic islet provides an accurate approach to identify biomarkers in the pancreas of diabetics. However, bulk RNA analysis can only deliver the mean abundance for each gene. Given that islet cell composition is highly variable even among non-diabetic controls , the high heterogeneity of individual cells is masked, leading to absence and failure of studies on potential therapeutic target . Since the advent of single-cell RNA sequencing (scRNA-seq) in 2009, which expanded our knowledge about biological systems , a dozen publication have reported results of scRNA-seq, providing insights into the heterogeneity of islet cells. Nevertheless, scRNA-seq is not yet possible to detect low-abundance transcripts due to limited efficiency of initial mRNA capture and cDNA conversion, which may lead to loss of valuable information and increased sampling errors . Therefore, a combination of bulk RNA-seq with scRNA-seq analysis may be a proper option for identifying new diabetes therapeutic targets.
In this study, CDKN1C and DLK1 were identified by intersected analysis of scRNA-seq datasets with bulk RNA-seq datasets, their alternation was verified in primary pancreatic islet cells cultured in high glucose. Besides, we forecasted the potential compounds targeting CDKN1C and DLK1 in T2DM, providing a theoretical basis for future studies on the mechanisms of T2DM progression and the exploitation of innovative therapies in future.
The single-cell landscape of human non-diabetic and T2DM
A total of 17 non-diabetic and 8 T2DM patient samples from the GSE81608 and GSE86469 datasets were covered in this study. Among them, 651 single cells were from non-diabetic (n = 12), while 949 were cells of T2DM (n = 5) origin in the GSE81608 dataset; in the GSE86469 dataset, 380 and 258 cells were extracted from non-diabetic (n = 5) and T2DM (n = 3) samples, respectively. After elimination of low-quality cells, a total of 2130 cells were finally included in the analysis, namely 622 cells in GSE81608-non-diabetic, 870 cells in GSE81608-T2DM (Fig. 1A), 380 cells in GSE86469-non-diabetic, and 258 cells in GSE86469-T2DM (Fig. 2A). PCA dimensionality reduction based on highly variable genes (k = 2000; Fig. 1B and 2B) was then performed for all cells in both datasets, respectively. PCA based on highly variable genes for all cells in each dataset (k = 2000; Figs. 1B and 2B) were then applied to affirm the available principal components (PCs) based on P < 0.05. Through the combinations of resolutions and PC value for the cluster identification, 15 PCs were selected in the GSE81608 dataset (Fig. 1C and Additional file 1: Fig. S1A), and 20 PCs were elected in the GSE86469 dataset (Fig. 2C and Additional file 1: Fig. S1B).
Then, the cells in GSE81608 were divided into 9 individual clusters (C0-C8; Fig. 1D) with the tSNE algorithm, and analogously, the cells in the GSE86469 dataset were partitioned into six primary cell lineages (C0-C5; Fig. 2D). Besides, Figs. 1E and 2E also illustrated the sample sources of each cluster in both datasets. Subsequently, 306 marker genes were characterized in the GSE81608 dataset-9 clusters by the FindAllMarkers function (Fig. 1F; Additional file 1: Table S1). A total of 649 marker genes from all 6 clusters (GSE86469 dataset) were authenticated (Fig. 2F; Additional file 1: Table S2).
Phenotyping of human non-diabetic and T2DM cells
Cell populations were annotated in the CellMarker database with the R package singl based on the expression patterns of the authenticated marker genes. In the GSE81608 dataset (Fig. 3A), C0, C1, C3, C4, and C8 were labeled as Neurons, C2 as Hepatocytes, C5 as Epithelial cells, C6 as Macrophage, and C7 as Smooth muscle cells. The six cell clusters from the GSE86469 dataset were illustrated as iPS cells (C0), neurons (C1, 2, and 3), epithelial cells (C4), and smooth muscle cells (C5) (Fig. 3B). Collectively, neurons, epithelial cells, and smooth muscle cells were the cell clusters annotated in both datasets, implying that they may potentially feature an essential role in non-diabetic and T2DM. Additionally, the cellular provenance of the three common cell clusters from both datasets is tabulated in Table 1.
The latent mechanism of common cell clusters in non-diabetic and T2DM
Cross-tabulation analysis was utilized to pinpoint identical marker genes in each common cell cluster from the GSE81608 and GSE86469 datasets. The results are presented in Fig. 3C. In the two datasets, there were 42 common-marker genes between neurons (noted as neurons’ marker genes; Additional file 1: Table S3), a total of 78 epithelial cells’ marker genes (Additional file 1: Table S4), and 84 smooth muscle cells’ marker genes (Additional file 1: Table S5).
Thereafter, functional annotation was performed against these marker genes to enlighten the utility of the common cell cluster in non-diabetes and T2DM. These genes contained a total of 554 BP terms, 58 CC terms, and 68 MF terms in the GO system (Additional file 1: Table S6). This study primarily concentrated on the results of BP categories (Fig. 3D). Specifically, neurons’ marker genes were mainly linked to T2DM-related processes, such as peptide/peptide hormone secretion, insulin secretion, and carbohydrate/sugar metabolism. Epithelial cells’ marker genes were associated with neutrophil activation, degranulation and their mediated immunity; predictably, epidermal/epithelial differentiation/migration/apoptosis processes were notably enriched; moreover, these genes were also closely affiliated with wound healing, hemostasis/coagulation, vascular development, and lipid localization/translocation. Smooth muscle cells’ marker genes were predominantly enriched in the extracellular matrix and structural tissues; consistently, they were also tightly integrated with wound healing, hemostasis/coagulation, and vascular development; strikingly, pancreatic development and B-type pancreatic cell proliferation terms were distinctly enriched; meanwhile, smooth muscle cell/epithelial cell migration and neuronal death/projection expansion were also detected. KEGG enrichment analysis (Fig. 3E; Additional file 1: Table S7) indicated that neurons’ marker genes were participating in insulin secretion, glucagon signaling pathway, and maturity-onset diabetes in young adults; epithelial cells’ marker genes and smooth muscle cells’ marker genes were strongly coupled to the most common vascular outcome of T2DM, coronary heart disease (CHD) , especially the epithelial cells' marker genes, which were linked to multiple CHD-related pathways (hypertrophic and dilated cardiomyopathy, atherosclerosis, arrhythmogenic right ventricular cardiomyopathy). Additionally, marker genes of smooth muscle cells were cited in the pathway of AGE-RAGE signaling in diabetic complications.
Pinpointing of aberrantly expressed marker genes in the common cell clusters
Next, the FindMarkers function (R package Seurat) was employed to analyze DEGs between non-diabetic and T2DM samples in the common cell cluster in GSE81608 (Additional file 1: Table S8) and GSE86469 (Additional file 1: Table S9) datasets. Then, robust DEMGs in neurons, epithelial cells, and smooth muscle cells cell clusters were investigated separately by using cross-tabulation analysis. As depicted in Fig. 4A, TTR, SST, CDKN1C, and DLK1 were DEMGs in the neurons cluster; SDC4 was a DE-marker gene in the epithelial cells cluster; no robust DE-marker gene was confirmed in the smooth muscle cells cluster.
Pseudo-time trajectory analysis of the common cells
To further explore the landscape of neurons, epithelial and smooth muscle cells in the non-diabetic and T2DM microenvironments, the differentiation trajectories of neurons, epithelial cells, and smooth muscle cells from both sources (non-diabetic and T2DM) were mimicked in the GSE86469 dataset.
Genes with abnormal expression in neurons, epithelial cells, and smooth muscle cells populations were authenticated between non-diabetic and T2DM samples. A dendrogram of differentiation trajectories of the 3 cell lineages through these genes was then constructed using the R package Monocle (Fig. 4B). Concerning cell typing, the initiation of the branches and nodes 2 and 3 consisted of neurons, while the other two branches (at node 1) mainly consisted of epithelial and smooth muscle cells and a few neurons (Fig. 4C). From the perspective of the original clusters of cells (cell cluster name not labeled), the initiation segment of differentiation mainly contained C1 and C2 cells, the middle segment was composed of C3, and the end segment contained all C4 and C5 and a few C3 cells (Fig. 4D). In terms of cell origin (from either non-diabetic or T2DM samples), cells from both non-diabetic and T2DM samples were distributed throughout the differentiation process (Fig. 4E).
Furthermore, variations in the expression of the five previously authenticated DEMGs during the differentiation of the three cell types were monitored. The DEMGs CDKN1C and DLK1 of the neurons cluster exhibited a slightly upregulated expression pattern from neuronal cells to epithelial and smooth muscle cells, followed by a gradual decrease; whereas SST displayed a trend of progressive up-regulation and then down-regulation during the differentiation of neuronal cells into epithelial and smooth muscle cells, and the levels of this gene were almost equilibrated at the initial and terminal ends of the differentiation; for TTR, generally, it presented a decrease expression during the process of cell differentiation; the SDC4 gene of the epithelial cells population manifested a relatively advanced expression at the end of differentiation (Additional file 1: Fig. S2).
Analysis of T2DM-related DEMGs
In the GSE86468 dataset, a total of 2098 DEGs linked to T2DM were authenticated by R package limma, using |log2 FC|> 0.5 and P < 0.05 as the significance threshold (Additional file 1: Table S10). Compared to non-diabetic patients (n = 15), 2058 genes were markedly upregulated in the T2DM (n = 9) population and 40 were downregulated in T2DM patients (Fig. 5A). The cross-tabulation analysis then indicated that CDKN1C, DLK1 and SDC4, among the five previously characterized DEMGs, were also T2DM-related DEGs (Fig. 5B), and they were defined as T2DM-related DEMGs that may not only be implicated in the pathogenesis of T2DM but also exert crucial functions in the common cell clusters.
GO analysis highlighted that these T2DM-related DEMGs in the BP category were intimately affiliated with exosome secretion and its regulatory processes; meanwhile, the Notch signaling pathway, cell–matrix adhesion, and regulatory processes of T cell proliferation/activation were notably enrolled; in addition, these genes were inextricably linked to urological development (mesonephric development, mesonephric tubule/epithelial development, and ureteral bud development). Furthermore, these genes were potentially responsible for regulating the activity and binding of cell cycle proteins, protein kinase regulators, and protein kinase inhibitors in the costamere. The top 10 BP entries and all CC and MF entries are visualized in Fig. 5C, and the detailed GO analysis results are provided in Additional file 1: Table S11. In KEGG analysis, these T2DM-related DEMGs were principally engaged in pathways relevant to inflammatory responses, cell cycle, atherosclerosis, and proteoglycans in cancer (Fig. 5D; Additional file 1: Table S12).
Assessment of the diagnostic value and clinical relevance of the T2DM-related DEMGs
To appraise the usability of the three T2DM-related DEMGs in recognizing T2DM and non-diabetic samples, ROC curve analysis was performed based on their expression profiles extracted from the GSE86468 and GSE29226 datasets, respectively. The results revealed that CDKN1C and DLK1 had favorable sample discrimination in both datasets (both AUC > 0.75; Fig. 6A) and considered as critical genes. The ROC results in GSE29221 confirmed the optimal predictive performances of these genes (both AUC > 0.7, Fig. 6A). Further, it was noticed that CDKN1C and DLK1, marker genes of neuronal cell clusters, were expressed differentially between non-diabetic and T2DM samples in epithelial cell clusters and smooth muscle cell clusters from GSE81608 and GSE86469 (Additional file 1: Fig. S3). Furthermore, using the Single Cell Expression Atlas database, it was also observed that the mRNA levels of two critical genes were significantly increased in type B pancreatic cells from T2DM patients (Additional file 1: Fig. S4A). On the other hand, the t-SNE map from the E-MTAB-5061 datasets indicated that the two critical genes expressed in both pancreatic endocrine cells and exocrine cells as well, such as acinar cells and ductal cells (Additional file 1: Fig. S4B).
Subsequently, the connection of CDKN1C and DLK1 with the clinical traits of T2DM was evaluated. The results revealed that in the ethnic subgroups (African American, Hispanic, and White), both genes possessed the highest expression in the Hispanic population and the lowest expression in the African American population (Fig. 6B); in the gender subgroup, DLK1 expression was considerably decreased in the male population compared with that in the female cohort, whereas CDKN1C was unremarkable with the gender of the patients (Fig. 6C). Relevance analysis confirmed that DLK1 expression levels were positively linked to patient age (r = 0.48, P = 0.02), whereas CDKN1C was irrelevant to this trait (r = 1.82e-03, P = 0.99; Fig. 6D); moreover, non-association of both genes with patient BMI was evident (Additional file 1: Fig. S5).
Exploration of the latent molecular mechanisms of the critical genes in the T2DM progression
An enrichment analysis using predefined gene sets revealed the possible molecular mechanisms of the critical genes in the T2DM process. For the critical gene CDKN1C, a total of 14 GO terms (Additional file 1: Table S13) and 3 KEGG pathways (Additional file 1: Table S14) were ensured. In Fig. 7A, B, the top 10 GO terms and all KEGG pathways were displayed, respectively. Collectively, CDKN1C was tightly integrated with keratinization, meiosis, and immune response. The analysis of GO terms for DLK1 (Fig. 7C; Additional file 1: Table S15) hinted that DLK1 might be directly tied to T2DM, and it was found that peptide/peptide hormone/insulin secretion and glucose/fatty acid processes were distinctly enriched; additionally, it was participating in responses to carbohydrates, metabolism of purine-containing compounds, and coagulation-related biological processes. The six KEGG pathways in which DLK1 was engaged are displayed in Fig. 7D (Additional file 1: Table S16).
Expression analysis of CDKN1C and DLK1 by western blotting
The expression of CDKN1C and DLK1 was further confirmed in rat pancreatic islet cells. Compared with control group, CDKN1C and DLK1 were upregulated after 30 mM glucose treatment for 48 h (Fig. 8).
Projection of the potential compounds targeting the critical genes
Key gene–compound interactions were predicted using the PubChem database. A total of 98 potential compounds capable of affecting CDKN1C expression were predicted in the premise of Homo sapiens (Additional file 1: Table S17), of which, 11 compounds (lactic acid, hydroquinone, nickel, beta-naphthoflavone, disulfiram, valproic acid, vorinostat, sulforaphane, pirinixic acid, estradiol, and cytarabine) were reported to decrease the expression of CDKN1C. Of the 41 potential compounds predicted to affect DLK1 expression (Additional file 1: Table S18), 21 compounds (2,2-bis(4-glycidyloxyphenyl)propane, benzo(a)pyrene, valproic acid, fluorouracil, entinostat, vorinostat, estradiol, sarin, tetrachlorodibenzodioxin, copper sulfate, doxorubicin, rosiglitazone, trichostatin a, tretinoin, thapsigargin, 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1h-imidazol-2-yl)benzamide, cyclosporine, sunitinib, panobinostat, dorsomorphin, and phenylmercuric acetate) were previously described to reduce DLK1 expression. Valproic acid, vorinostat, and estradiol were discovered to simultaneously downregulate the expression of two critical genes.
The prevalence of diabetes mellitus is 9% worldwide, with type 2 diabetes (T2DM) accounting for 90% of all cases . However, the underlying mechanisms of T2DM are poorly understood as yet. Although lifestyle and diet partially account for this health disparity, more than 100 robust association signals in T2DM have been identified in genome-wide association studies [10, 11], indicating the complex polygenic nature of T2DM. Thus, identification of the core genes may provide potential target for the treatment of T2DM. The pancreatic islet cells play an important role in the development of diabetes. The tremendous advance in transcriptomic analysis of single cells over the past five years has contributed to a better understanding of the role of islet cell-associated genes in the pathogenesis of diabetes. Given that scRNA-seq offers a unique perspective on the mechanism driving responses within and outside of the disease, it allows us to focus on cell alterations . CDKN1C and DLK1 of T2DM were identified by integrated analysis of scRNA-seq and bulk RNA-Seq in this study. They are further linked to the clinical characteristics of T2DM patients by internal and external validation. Additionally, the potential compounds targeting CDKN1C and DLK1 in T2DM are forecasted and profiled. Our study provides a theoretical basis for investigating the mechanisms of T2DM progression and exploiting innovative therapies in the future.
In islet cells, endocrine cells have been shown to be identified by the expression of INS (beta cell), GCG (alpha cell), SST (delta cell), PPY (PP cell), GHRL (epsilon cell), while exocrine cells by COL1A1 (stellate cells), PRSS1 (acinar cells) and KRT19 (ductal cells) [12,13,14]. However, the genetic alternation of others cells such as neurons, epithelial cells and smooth muscle cells have not received sufficient attention. Indeed, while the autonomic nervous system innervates beta, alpha, and delta cells in the islet of mouse, the sympathetic fibers preferentially innervate smooth muscle cells in the blood vessels and control hormone secretion in human islets by affecting local blood flow instead of modulating endocrine cell function directly . In this study, cell cluster have been annotated by CellMarker database and neurons, epithelial cells and smooth muscle cells have been identified in both datasets. As expected, marker genes of neurons are mainly linked to T2DM-related processes, such as peptide/peptide hormone secretion and insulin secretion, suggesting a crucial role of neurons in control of hormone secretion. Actually, the degree of islets innervation is three times greater in patients with T2DM than in controls, and the increase in fibers is negatively correlated with insulin secretion and glucose tolerance . Moreover, HMG20A, a chromatin factor involved in neuronal differentiation and maturation, has been reported for metabolism–insulin secretion coupling. What’s more, the functional consequence of T2DM-linked rs7119 SNP reducing HMG20A expression may lead to impaired beta cell mature function . In addition to neuron alternations, marker genes in epithelial and smooth muscle cells are associated with immunity, wound healing, barrier function and CHD-related pathway, indicating that the inflammation and vascular injury may also be potential mechanism of T2DM in islet. Hence, accumulating evidences have shown that targeting islet inflammation may be an effective therapeutic strategy for T2MD patients .
CDKN1C, located on the human chromosomal band 11 p15.5, encodes the cyclin-dependent kinase inhibitor 1c (p57, Kip2), which is known as “a tumor suppressor gene” [19, 20]. It not only negatively regulates cell proliferation, but also directs the differentiation of certain select lineages, as well as maintains adult quiescent neural stem cell populations of adult quiescent . Previous studies confirmed that CDKN1C played an important role in the neurogenesis, migration and morphology , of which overexpression could inhibit the proliferation of β-cells, leading to diabetes . As indicated by the enrichment analysis, CDKN1C enriches in biological processes of keratinization, meiosis, and immune response. Expression levels of keratin 17 protein, which is involved in the keratinization pathway, are confirmed to be upregulated in T2DM . Previous studies have shown that children of mothers with gestation diabetes are more likely to become obese and develop diabetes in adulthood, suggesting that meiosis may play a critical role in the development and progression of T2DM . Dysregulation of adaptive immune cells may have relevance to T2DM and its comorbidities [25, 26]. Compared with control group, we found that the expression of CDKN1C was increased in primary pancreatic islet cells cultured with 30 mM glucose. In addition, the correlation with clinical characteristics has been validated with both internal and external datasets, suggesting that it may contribute to T2DM.
Delta-Like 1 Homolog (DLK1), a transmembrane protein to the Notch/Delta/Serrate family , is paternally expressed and belongs to a group of imprinted genes located on chromosome band 14q32 in humans and 12qF1 in mice . It is expressed in many human tissues during embryonic development but is low in adults expression and is mostly restricted to (neuro)endocrine tissues and other immature stem/progenitor cells (notably hepatoblasts) , such as normal pituitary gland, spinal cord, pancreatic islet cells, adrenals, and Leydig cells . Accumulating evidences demonstrate that DLK1 plays an important role in energy metabolism  and exerts neuroendocrine effects . In addition to inhibiting adipocyte differentiation , it also determines the cell fate of pancreatic islet cells and neurons [33, 34]. DLK1 is involved in the differentiation of pancreatic ductal cells into β-cells, and promotes insulin synthesis and secretion by activating AKT signaling [35,36,37]. However, animal studies have shown that mice overexpressing DLK1 are insulin resistant . Our enrichment analysis also demonstrated that DLK1 was significantly upregulated in pathways including cellular glucose homeostasis, insulin secretion, mitochondrial matrix and glutathione metabolism. There is a general consensus that T2DM is characterized by chronic hyperglycemia resulting from impaired insulin secretion  and altered glutathione metabolism , and mitochondrial dysfunction in adipose tissue partially participates in the pathogenesis of T2DM . Compared with control group, the expression of DLK1 was increased in primary pancreatic islet cells cultured with 30mM glucose. Combined its correlation with clinical characteristics validated by internal and external datasets, DLK1 is expected to become a therapeutic target of T2DM . However, DLK1 is also associated with paternally inherited risk of type 1 diabetes, because the influence of SNP rs941576, which is in the imprinted region of chromosome 14q32.2 and at 105 kb downstream of DLK1 . Hence, further studies need to be performed to detect its role in different types of diabetes.
Notably, we predicted that 3 compounds could down-modulate CDKN1C and DLK1 simultaneously. Valproic Acid (VPA), a histone deacetylase inhibitor, is widely applied in the treatment of bipolar disorder. In human embryonic stem cell (hESC)- based in vitro systems for developmental neurotoxicity and reproductive toxicity testing, VPA is used as a positive control compound to treat H9 hESCs. It can decrease the expression of DLK1 in JRC and UKN1 system and the expression of CDKN1C in UKK system . Vorinostat, another histone deacetylase inhibitor, also suppresses the expression of CDKN1C and DLK in UKN1 system by intervening H9 hESCs [45, 46]. Hence, VPA and vorinostat might be effective in reversing aforementioned islets deficits in T2DM, such as hormone secretion, inflammation and vascular injury, which remain to be verified in the future. In addition, CDKN1C and DLK1 appear to be hormonally regulated, with the former decreasing after expose to estradiol in endometrial cancer cells , while the latter decreasing after treatment with estradiol in girls with anorexia nervosa or human uterine leiomyoma cells [48, 49]. Since the level of estradiol is correlated with age, especially for female, it is understandable why the expression of DLK1 varies by gender and is positively correlated with age, albeit the underlying mechanism needs further investigation.
However, there were some limitations in this study. Further functional experiments based on knockout mice and large sample cohort studies are needed to confirm the regulatory mechanisms targeting these genes in various cell types of the human pancreas.
In summary, we identified two core genes in T2DM though integrative analysis of two scRNA-seq dataset and a bulk RNA-seq dataset and validated their alternation in primary pancreatic islet cells cultured with or without high glucose. Moreover, the compounds associated with these key genes were predicted for further analysis in T2DM.
Materials and methods
The single-cell sequencing data (non-diabetic and T2DM patients) in our study were freely retrieved from the GEO database under accession numbers GSE86469 and GSE81608 which were generated using the same protocol: Smart-Seq RNA-sequencing method combined with by Fluidigm C1 system (SMARTer/C1) . The GSE86469 dataset  contains 638 individual islet/other single-cell RNA-sequencing data from 5 non-diabetic and 3 T2DM samples (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86469). The GSE81608 dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE81608) encompasses a total of 1600 single-cell (islet) sequencing data from 12 non-diabetic samples and 5 T2DM samples . The cell volume statistics for each type of sample comprised in the GSE86469 and GSE81608 datasets are presented in Table 2. And meanwhile, single-cell RNA-sequencing data from E-MTAB-5061 (contained the pancreatic tissue and islets from six non-diabetic samples and four T2DM samples) (https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-5061?query=E-MTAB-5061) was downloaded from ArrayExpress (EBI) to explore the expression profiles of target genes in endocrine and exocrine cell types of the human pancreas . Further, the GSE86468 and GSE29226 datasets were prepared to screen the target genes and estimate the diagnostic value of which supplemented by external validation set GSE29221. The GSE86468 dataset (platform: GPL18573) (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86468) entailed RNA-seq profiles of 24 individual islet/other bulk cell samples (obtained from 15 non-diabetic and 9 T2DM cadaveric organ donors) . The GSE29226 dataset  (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE29226) holds gene expression profiles based on 24 subcutaneous fat biopsies (three biological replicates and four technical replicates) from three T2DM patients and three non-diabetic patients (all female), which were produced with the Illumina HumanHT-12 v3 Expression BeadChip array (GPL6947 platform). The GSE29221 dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE29221) was generated on the platform of GPL6947 with the Illumina HumanHT-12 V3.0 expression beadchip and contained the transcription data from three T2DM and three non-diabetic individuals as per four times.
Exclusion of the low-quality cells
The comprehensive analysis of single-cell sequencing data in this study was initiated through the ‘IntegrateData’ functions within R package Seurat (version 4.1.0) based on the GSE86469 and GSE81608 datasets, respectively. The exclusion criteria for low-quality cells in this study are as follows: (1) the number of detectable genes in a single cell was less than 100; (2) the proportion of mitochondrial genes in a single cell is ≥ 5%; and (3) the number of genes detected in a single cell is ≤ 3. Besides, genes that failed to detect expression in up to 3 single cells would be discarded. Eventually, in the GSE81608 dataset, we gained 1492 cells (622 derived from non-diabetic patients and 870 from T2DM samples) and 28134 genes. No low-quality cells were recognized in the GSE86469 dataset of 638 cells (non-diabetic and T2DM samples contained 380 and 258 cells, respectively), while 17280 genes from this dataset were captured in the follow-up analysis.
Reduced-dimensional and categorization analysis
The ‘FindVariableFeatures’ function (vst method) was conducted to calculate feature variance on the standardized values after clipping to a maximum and select the high variability genes (top 2000) for downscaling on the filtered retained core cells. Next, the consistency of the sample distribution was monitored by principal component analysis (PCA). In this process, PCs with P < 0.05 were recognized for subsequently unsupervised cluster analysis (that is, K-means clustering algorithm) , where a single-cell resolution was combined with PCs in elbow plot to determine relevant sources of heterogeneity. Finally, the results of the unsupervised clustering analysis were visualized in nonlinear t-SNE  plots. The above analyses were executed in both GSE86469 and GSE81608 datasets.
Notation of the cell subpopulation types
Following the findings of tSNE, the marker genes for each cell subpopulation were located in every dataset by utilizing the FindAllMarkers function. The parameters were set to min.pct = 0.5, logfc.threshold = 1, min.diff.pct = 0.3, and p_val < 0.05. Cell cluster phenotypes were determined by comparing marker genes of each cluster in the CellMarker database (SingleR package, version 1.6.1). The cell phenotypes which were advertised in both datasets were principally focused and denominated as the common cell clusters to ensure the robustness of the identified clusters [54, 55].
Functional elucidation of the marker genes
The shared marker genes from each common cell cluster in both datasets (achieved by cross-tabulation analysis) were selected to implement a functional interpretation, aiming to preliminarily probe the latent mechanisms of each cell cluster in non-diabetic and T2DM. The analysis was undertaken in the R package clusterProfiler based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The enrichment criterion was established as P < 0.05. The cross-tabulations and the Venn diagrams presenting the results were executed in the online tool Jvenn, at the URL http://jvenn.toulouse.inra.fr/app/example.html.
Pinpointing the aberrantly expressed marker genes from non-diabetic and T2DM cells within the common cell cluster
Differentially expressed genes (DEGs) between non-diabetic and T2DM cells were also characterized, respectively, in the common cell clusters of the corresponding datasets, and analyzed by the FindMarkers function of the R package Seurat. The threshold was set to |average log2-fold change (FC)|≥ 0.5 and P ≤ 0.05. Next, Differentially expressed marker genes (DEMGs) representing the corresponding common cell clusters were elicited by the crossover of marker genes from a single common cell cluster and DEGs within a single common cell cluster in both datasets.
Putative time-series analysis of the common cell clusters
The differentiation trajectories and processes of the 3 common cell clusters were abstracted using Monocle . Briefly, signature genes were screened in the common cell cluster using Seurat with the following criteria: (1) expression detected in no less than 10 cells; (2) |mean expression value|> 0.5; and (3) differential expression analysis q < 0.01. Then, pseudo-time-series analysis was undertaken by R package Monocle based on the authenticated signature genes. Eventually, a pseudo-time-series trajectory chart of the common cell phenotypes was visualized using the plot_cell_trajectory function.
The variability of gene expression levels between the T2DM group and the non-diabetic group was compared by R package edgeR in the GSE86468 dataset, with |log2 FC|> 0.5 and P < 0.05 set as the threshold for significant differences. Besides, intersection analysis was utilized to recognize DEMGs in the GSE86468-DEGs, defined as T2DM-related DEMGs, and R package clusterProfiler-based functional annotation was implemented for them (see 2.5 for details).
ROC curve-based assessment of diagnostic benefit
The area under curve (AUC) of the receiver operating characteristic curve (ROC) was applied to appraise the usability of the ascertained T2DM-related DEMGs in diagnostic non-diabetic and T2DM samples. Briefly, the expression profiles of the T2DM-related DEMGs in the GSE86468 and GSE29226 datasets were matched, the ROC curves of the corresponding genes were plotted and the AUCs were calculated by R package pROC in both datasets . Only genes with AUCs greater than 0.75 in both datasets were admitted in the ensuing analysis here. These genes were defined as “critical genes”. Further, the predictive performance of critical genes was validated by a external validation set (GSE29221).
Enrichment analysis of pre-specified gene sets of the critical genes
Elucidating the hidden functions of the critical genes may facilitate the interpretation of the progression of T2DM. Here, the corresponding subsets (c5.go.v7.4.entrez.gmt; c2.cp.kegg.v7.4.entrez.gmt) were used as preset gene sets, which were freely available from the Molecular Signatures Database (MSigDB) [58, 59], URL: http://www.broadinstitute.org/msigdb. The samples were divided into high and low risk groups based on the median expression of the single key gene. Gene Set Enrichment Analysis (GSEA) was carried out by using R package clusterProfiler in the high and low risk groups in the T2DM cohort (GSE86468 dataset). Worthy terms/pathways were ascertained by |Normalized Enrichment Score (NES)|> 1, P < 0.05, and q < 0.25.
Primary pancreatic islet cells (CP-R015) were purchased from Procell Life Science & Technology Co.,Ltd. They were cultured with or without 30 mM glucose (high glucose, HG) for 48 h. Protein concentrations were extracted using ice-cold RIPA buffer (Beyotime, Nantong, China) containing protease inhibitor and phosphatase inhibitor (Thermo Fisher Scientific, Waltham, MA, USA). A 20–30 μg protein was subjected to 10–15% SDS polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (PVDF, Millipore, Bedford, MA, USA). The PVDF membranes were blocked in 5% skim milk for 1h and then incubated overnight at 4 ℃ with DLK1(sc-376755, Santa Cruz Biotechnology) or CDKN1C (sc-56341, Santa Cruz Biotechnology). After incubating with appropriate secondary antibodies for 2 h, the band densities were determined using Image J software (NIH, Maryland, USA) and normalized to each internal control.
Forecasting and profiling of the potential compounds targeting the critical genes in T2DM
The interaction of compounds with critical genes in the PubChem database (https://pubchem.ncbi.nlm.nih.gov/) was pretested by implementing "drug–gene interactions" (default values were selected for parameters). In conjunction with the expression trend of critical genes in T2DM (relative to the non-diabetic group), compounds that could override this expression trend were suggested as the potential compounds for targeting the critical genes.
Expression patterns of critical genes in single-cell-related datasets
Considering the significance of gene expressions in different cell types in T2MD and non-diabetic individuals, cells in GSE81608 and external E-MTAB-5061 datasets were further annotated with the help of islet tissue marker genes in the Single Cell Expression Atlas (https://www.ebi.ac.uk/gxa/sc/home) database to analysis the expressed difference of critical genes between T2DM group and non-diabetic group, including pancreatic A cell: TTR, GCG, CFC1, PCSK2, SLC38A4; pancreatic D cell: SST, LEPR, RBP4, SEC11C, HHEX; pancreatic PP cell: PPY, PPY2P, MEIS2, ETV1, ABCC9; pancreatic ductal cell: KRT19, CFTR, ANXA4, CLDN1, KRT8; pancreatic stellate cell: COL1A1, CALD1, SPARC, BGN, SERPINH1; type B pancreatic cell: INS, INS-IGF2, HADH, ADCYAP1, IAPP.
The differences in the critical gene expression between samples (non-diabetic and T2DM samples) were detected by the Wilcoxon rank-sum test at P < 0.05, as were the detection methods and thresholds between different clinical characteristic subtypes (race and gender). The correlations between critical genes and patients' BMI and age were illuminated by Pearson correlation analysis, with remarkable correlations defined by | correlation coefficient (r)|> 0.3 and P < 0.05. The software packages and statistical analyses addressed in this study were exhaustively described in the corresponding positions. The difference of western blotting analysis was detected by t-test under the help of GraphPad Prism software (GraphPad Software, version 7.0). P value lower than 0.05 (p < 0.05) was considered as significant differences.
Availability of data and materials
The datasets [GSE86469, GSE81608, GSE86468, GSE29226 and GSE29221] for this study can be found in the GEO database (https://www.ncbi.nlm.nih.gov/geo/) and ArrayExpress (EBI) (https://www.ebi.ac.uk/biostudies/arrayexpress/studies/). The related code for present study could be found on GitHub at https://github.com/liyansong1987/code-for-manuscript.
Jin C, Chen S, Vaidya A, Wu Y, Wu Z, Hu F, Kris-Etherton P, Wu S, Gao X. Longitudinal change in fasting blood glucose and myocardial infarction risk in a population without diabetes. Diabetes Care. 2017;40(11):1565–72.
Valencia WM, Florez H. How to prevent the microvascular complications of type 2 diabetes beyond glucose control. BMJ. 2017;356:i6505.
Seuring T, Archangelidi O, Suhrcke M. The economic costs of Type 2 diabetes: a global systematic review. Pharmacoeconomics. 2015;33(8):811–31.
Association AD. Classification and diagnosis of diabetes: standards of medical care in diabetes-2021. Diabetes Care. 2021;44(Suppl 1):S15-s33.
Wang Y, Golson M, Schug J, Traum D, Liu C, Vivek K, Dorrell C, Naji A, Powers A, Chang K, Grompe M, Kaestner K. Single-cell mass cytometry analysis of the human endocrine pancreas. Cell Metab. 2016;24(4):616–26.
Wang YJ, Kaestner KH. Single-cell RNA-Seq of the pancreatic islets-a promise not yet fulfilled? Cell Metab. 2019;29(3):539–44.
Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch B, Siddiqui A, Lao K, Surani M. mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods. 2009;6(5):377–82.
Rao Kondapally SS, Kaptoge S, Thompson A, Di Angelantonio E, Gao P, Sarwar N, Whincup P, Mukamal K, Gillum R, Holme I, Njølstad I, Fletcher A, Nilsson P, Lewington S, Collins R, Gudnason V, Thompson S, Sattar N, Selvin E, Hu F, Danesh J. Diabetes mellitus, fasting glucose, and risk of cause-specific death. N Engl J Med. 2011;364(9):829–41.
Neuenschwander M, Barbaresko J, Pischke C, Iser N, Beckhaus J, Schwingshackl L, Schlesinger S. Intake of dietary fats and fatty acids and the incidence of type 2 diabetes: a systematic review and dose-response meta-analysis of prospective observational studies. PLoS Med. 2020;17(12):1003347.
Estrada K, Aukrust I, Bjørkhaug L, Burtt N, Mercader J, García-Ortiz H, Huerta-Chagoya A, Moreno-Macías H, Walford G, Flannick J, Williams A, Gómez-Vázquez M, Fernandez-Lopez J, Martínez-Hernández A, Jiménez-Morales S, Centeno-Cruz F, Mendoza-Caamal E, Revilla-Monsalve C, Islas-Andrade S, Córdova E, Soberón X, González-Villalpando M, Henderson E, Wilkens L, Le Marchand L, Arellano-Campos O, Ordóñez-Sánchez M, Rodríguez-Torres M, Rodríguez-Guillén R, Riba L, Najmi L, Jacobs S, Fennell T, Gabriel S, Fontanillas P, Hanis C, Lehman D, Jenkinson C, Abboud H, Bell G, Cortes M, Boehnke M, González-Villalpando C, Orozco L, Haiman C, Tusié-Luna T, Aguilar-Salinas C, Altshuler D, Njølstad P, Florez J, MacArthur D. Association of a low-frequency variant in HNF1A with type 2 diabetes in a Latino population. JAMA. 2014;311(22):2305–14.
Zheng Y, Ley S, Hu F. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol. 2018;14(2):88–98.
Xin Y, Kim J, Okamoto H, Ni M, Wei Y, Adler C, Murphy A, Yancopoulos G, Lin C, Gromada J. RNA sequencing of single human islet cells reveals type 2 diabetes genes. Cell Metab. 2016;24(4):608–15.
Segerstolpe A, Palasantza A, Eliasson P, Andersson EM, Andreasson AC, Sun XY, Picelli S, Sabirsh A, Clausen M, Bjursell MK, Smith DM, Kasper M, Ammala C, Sandberg R. Single-cell transcriptome profiling of human pancreatic islets in health and type 2 diabetes. Cell Metab. 2016;24(4):593–607.
Lawlor N, George J, Bolisetty M, Kursawe R, Sun L, Sivakamasundari V, Kycia I, Robson P, Stitzel M. Single-cell transcriptomes identify human islet cell signatures and reveal cell-type-specific expression changes in type 2 diabetes. Genome Res. 2017;27(2):208–22.
Rodriguez-Diaz R, Abdulreda MH, Formoso AL, Gans I, Ricordi C, Berggren PO, Caicedo A. Innervation patterns of autonomic axons in the human endocrine pancreas. Cell Metab. 2011;14(1):45–54.
Cinti F, Mezza T, Severi I, Suleiman M, Cefalo CMA, Sorice GP, Moffa S, Impronta F, Quero G, Alfieri S, Mari A, Pontecorvi A, Marselli L, Cinti S, Marchetti P, Giaccari A. Noradrenergic fibers are associated with beta-cell dedifferentiation and impaired beta-cell function in humans. Metabolism. 2021;114:154414.
Mellado-Gil JM, Fuente-Martín E, Lorenzo PI, Cobo-Vuilleumier N, López-Noriega L, Martín-Montalvo A, Gómez IDGH, Ceballos-Chávez M, Gómez-Jaramillo L, Campos-Caro A, Romero-Zerbo SY, Rodríguez-Comas J, Servitja J-M, Rojo-Martinez G, Hmadcha A, Soria B, Bugliani M, Marchetti P, Bérmudez-Silva FJ, Reyes JC, Aguilar-Diosdado M, Gauthier BR. The type 2 diabetes-associated HMG20A gene is mandatory for islet beta cell functional maturity. Cell Death Dis. 2018;9(3):279.
Eguchi K, Nagai R. Islet inflammation in type 2 diabetes and physiology. J Clin Invest. 2017;127(1):14–23.
Lai J, Lin X, Cao F, Mok H, Chen B, Liao N. CDKN1C as a prognostic biomarker correlated with immune infiltrates and therapeutic responses in breast cancer patients. J Cell Mol Med. 2021;25(19):9390–401.
McNamara GI, Davis BA, Browne M, Humby T, Dalley JW, Xia J, John RM, Isles AR. Dopaminergic and behavioural changes in a loss-of-imprinting model of Cdkn1c. Genes Brain Behav. 2018;17(2):149–57.
Van de Pette M, Tunster SJ, John RM. Loss of imprinting of Cdkn1c protects against age and diet-induced obesity. Int J Mol Sci. 2018;19(9):E2734.
Ou K, Yu M, Moss NG, Wang YJ, Wang AW, Nguyen SC, Jiang C, Feleke E, Kameswaran V, Joyce EF, Naji A, Glaser B, Avrahami D, Kaestner KH. Targeted demethylation at the CDKN1C/p57 locus induces human β cell replication. J Clin Investig. 2018;129(1):209–14.
Kulkarni M, Leszczynska A, Wei G, Winkler MA, Tang J, Funari VA, Deng N, Liu Z, Punj V, Deng SX, Ljubimov AV, Saghizadeh M. Genome-wide analysis suggests a differential microRNA signature associated with normal and diabetic human corneal limbus. Sci Rep. 2017;7(1):3448.
Valladares-Salgado A, Suárez-Sánchez F, Burguete-García AI, Cruz M. Epigenetics of childhood obesity and diabetes. Rev Med Inst Mex Seguro Soc. 2014;52(Suppl 1):S88–93.
Zhou T, Hu Z, Yang S, Sun L, Yu Z, Wang G. Role of adaptive and innate immunity in type 2 diabetes mellitus. J Diabetes Res. 2018;2018:7457269.
SantaCruz-Calvo S, Bharath L, Pugh G, SantaCruz-Calvo L, Lenin RR, Lutshumba J, Liu R, Bachstetter AD, Zhu B, Nikolajczyk BS. Adaptive immune cells shape obesity-associated type 2 diabetes mellitus and less prominent comorbidities. Nat Rev Endocrinol. 2022;18(1):23–42.
Sakajiri S, O’Kelly J, Yin D, Miller CW, Hofmann WK, Oshimi K, Shih LY, Kim KH, Sul HS, Jensen CH, Teisner B, Kawamata N, Koeffler HP. Dlk1 in normal and abnormal hematopoiesis. Leukemia. 2005;19(8):1404–10.
Pittaway JFH, Lipsos C, Mariniello K, Guasti L. The role of delta-like non-canonical Notch ligand 1 (DLK1) in cancer. Endocr Relat Cancer. 2021;28(12):R271–87.
Villanueva C, Jacquier S, de Roux N. DLK1 is a somato-dendritic protein expressed in hypothalamic arginine-vasopressin and oxytocin neurons. PLoS ONE. 2012;7(4):e36134.
Bassatne A, Jafari A, Kassem M, Mantzoros C, Rahme M, El-Hajj Fuleihan G. Delta-like 1 (DLK1) is a possible mediator of vitamin D effects on bone and energy metabolism. Bone. 2020;138:115510.
Laborda J, Sausville EA, Hoffman T, Notario V. Dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line. J Biol Chem. 1993;268(6):3817–20.
Smas CM, Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell. 1993;73(4):725–34.
Carlsson C, Tornehave D, Lindberg K, Galante P, Billestrup N, Michelsen B, Larsson LI, Nielsen JH. Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas. Endocrinology. 1997;138(9):3940–8.
Montalbán-Loro R, Lassi G, Lozano-Ureña A, Perez-Villalba A, Jiménez-Villalba E, Charalambous M, Vallortigara G, Horner AE, Saksida LM, Bussey TJ, Trejo JL, Tucci V, Ferguson-Smith AC, Ferrón SR. Dosage regulates hippocampal neurogenesis and cognition. Proc Natl Acad Sci U S A. 2021;118(11):e2015505118.
Gomes LG, Cunha-Silva M, Crespo RP, Ramos CO, Montenegro LR, Canton A, Lees M, Spoudeas H, Dauber A, Macedo DB, Bessa DS, Maciel GA, Baracat EC, Jorge AAL, Mendonca BB, Brito VN, Latronico AC. DLK1 Is a novel link between reproduction and metabolism. J Clin Endocrinol Metab. 2019;104(6):2112–20.
Rhee M, Lee S-H, Kim J-W, Ham D-S, Park H-S, Yang HK, Shin J-Y, Cho J-H, Kim Y-B, Youn B-S, Sul HS, Yoon K-H. Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells. Sci Rep. 2016;6:23960.
Lee YH, Yun MR, Kim HM, Jeon BH, Park BC, Lee BW, Kang ES, Lee HC, Park YW, Cha BS. Exogenous administration of DLK1 ameliorates hepatic steatosis and regulates gluconeogenesis via activation of AMPK. Int J Obes (Lond). 2016;40(2):356–65.
Villena JA, Choi CS, Wang Y, Kim S, Hwang Y-J, Kim Y-B, Cline G, Shulman GI, Sul HS. Resistance to high-fat diet-induced obesity but exacerbated insulin resistance in mice overexpressing preadipocyte factor-1 (Pref-1). Diabetes. 2008;57(12):3258–66.
Rorsman P, Ashcroft FM. Pancreatic β-cell electrical activity and insulin secretion: of mice and men. Physiol Rev. 2018;98(1):117–214.
Hussain T, Tan B, Murtaza G, Liu G, Rahu N, Saleem Kalhoro M, Hussain Kalhoro D, Adebowale TO, Usman Mazhar M, Rehman ZU, Martínez Y, Akber Khan S, Yin Y. Flavonoids and type 2 diabetes: evidence of efficacy in clinical and animal studies and delivery strategies to enhance their therapeutic efficacy. Pharmacol Res. 2020;152:104629.
Kusminski CM, Ghaben AL, Morley TS, Samms RJ, Adams AC, An Y, Johnson JA, Joffin N, Onodera T, Crewe C, Holland WL, Gordillo R, Scherer PE. A novel model of diabetic complications: adipocyte mitochondrial dysfunction triggers massive β-cell hyperplasia. Diabetes. 2020;69(3):313–30.
Jensen CH, Kosmina R, Rydén M, Baun C, Hvidsten S, Andersen MS, Christensen LL, Gastaldelli A, Marraccini P, Arner P, Jørgensen CD, Laborda J, Holst JJ, Andersen DC. The imprinted gene Delta like non-canonical notch ligand 1 (Dlk1) associates with obesity and triggers insulin resistance through inhibition of skeletal muscle glucose uptake. EBioMedicine. 2019;46:368–80.
Wallace C, Smyth DJ, Maisuria-Armer M, Walker NM, Todd JA, Clayton DG. The imprinted DLK1-MEG3 gene region on chromosome 14q32.2 alters susceptibility to type 1 diabetes. Nat Genet. 2010;42(1):68–71.
Krug AK, Kolde R, Gaspar JA, Rempel E, Balmer NV, Meganathan K, Vojnits K, Baquie M, Waldmann T, Ensenat-Waser R, Jagtap S, Evans RM, Julien S, Peterson H, Zagoura D, Kadereit S, Gerhard D, Sotiriadou I, Heke M, Natarajan K, Henry M, Winkler J, Marchan R, Stoppini L, Bosgra S, Westerhout J, Verwei M, Vilo J, Kortenkamp A, Hescheler JR, Hothorn L, Bremer S, van Thriel C, Krause KH, Hengstler JG, Rahnenfuhrer J, Leist M, Sachinidis A. Human embryonic stem cell-derived test systems for developmental neurotoxicity: a transcriptomics approach. Arch Toxicol. 2013;87(1):123–43.
Rempel E, Hoelting L, Waldmann T, Balmer N, Schildknecht S, Grinberg M, Das Gaspar J, Shinde V, Stöber R, Marchan R, van Thriel C, Liebing J, Meisig J, Blüthgen N, Sachinidis A, Rahnenführer J, Hengstler J, Leist M. A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors. Arch Toxicol. 2015;89(9):1599–618.
Shinde V, Hoelting L, Srinivasan S, Meisig J, Meganathan K, Jagtap S, Grinberg M, Liebing J, Bluethgen N, Rahnenführer J, Rempel E, Stoeber R, Schildknecht S, Förster S, Godoy P, van Thriel C, Gaspar J, Hescheler J, Waldmann T, Hengstler J, Leist M, Sachinidis A. Definition of transcriptome-based indices for quantitative characterization of chemically disturbed stem cell development: introduction of the STOP-Tox and STOP-Tox tests. Arch Toxicol. 2017;91(2):839–64.
Gertz J, Reddy T, Varley K, Garabedian M, Myers R. Genistein and bisphenol A exposure cause estrogen receptor 1 to bind thousands of sites in a cell type-specific manner. Genome Res. 2012;22(11):2153–62.
Yu L, Moore A, Castro L, Gao X, Huynh H, Klippel M, Flagler N, Lu Y, Kissling G, Dixon D. Estrogen regulates MAPK-related genes through genomic and Nongenomic interactions between IGF-I receptor tyrosine kinase and estrogen receptor-Alpha Signaling pathways in human uterine leiomyoma cells. J Signal Transduct. 2012;2012:204236.
Faje A, Fazeli P, Katzman D, Miller K, Breggia A, Rosen C, Mendes N, Misra M, Klibanski A. Inhibition of Pref-1 (preadipocyte factor 1) by oestradiol in adolescent girls with anorexia nervosa is associated with improvement in lumbar bone mineral density. Clin Endocrinol (Oxf). 2013;79(3):326–32.
Lin Y, Cao Y, Kim HJ, Salim A, Speed TP, Lin DM, Yang P, Yang JYH. scClassify: sample size estimation and multiscale classification of cells using single and multiple reference. Mol Syst Biol. 2020;16(6):e9389.
Jain P, Vig S, Datta M, Jindel D, Mathur AK, Mathur SK, Sharma A. Systems biology approach reveals genome to phenome correlation in type 2 diabetes. PLoS One. 2013;8(1):16.
Lall S, Sinha D, Bandyopadhyay S, Sengupta D. Structure-aware principal component analysis for single-cell RNA-seq data. J Comput Biol. 2018;25(12):1365–73.
Satija R, Farrell JA, Gennert D, Schier AF, Regev A. Spatial reconstruction of single-cell gene expression data. Nat Biotechnol. 2015;33(5):495-U206.
Clarke ZA, Andrews TS, Atif J, Pouyabahar D, Innes BT, MacParland SA, Bader GD. Tutorial: guidelines for annotating single-cell transcriptomic maps using automated and manual methods. Nat Protoc. 2021;16(6):2749–64.
Park J, Shrestha R, Qiu C, Kondo A, Huang S, Werth M, Li M, Barasch J, Suszták K. Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease. Science. 2018;360(6390):758–63.
Qiu X, Mao Q, Tang Y, Wang L, Chawla R, Pliner H, Trapnell C. Reversed graph embedding resolves complex single-cell trajectories. Nat Methods. 2017;14(10):979–82.
Robin X, Turck N, Hainard A, Tiberti N, Lisacek F, Sanchez J, Müller M. pROC: an open-source package for R and S+ to analyze and compare ROC curves. BMC Bioinformatics. 2011;12:77.
Liberzon A, Birger C, Thorvaldsdóttir H, Ghandi M, Mesirov J, Tamayo P. The molecular signatures database (MSigDB) hallmark gene set collection. Cell Syst. 2015;1(6):417–25.
Liberzon A, Subramanian A, Pinchback R, Thorvaldsdóttir H, Tamayo P, Mesirov J. Molecular signatures database (MSigDB) 30. Bioinformatics. 2011;27(12):1739–40.
This study was supported by the National Natural Science Foundation of China (Grant Nos. 81974540, 82274290 and 81971290), Key Research & Development Program of Shaanxi (Program No. 2022ZDLSF02-09), and Innovation Capability Support Program of Shaanxi (Program No. 2021TD-58), Natural Science Basic Research Program of Shaanxi (Program No. 2022JQ-819 and 2023-JC-QN-0928).
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All animal studies (including the mice euthanasia procedure) were approved by Animal Care Institution of Xi’an Jiaotong University.
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: Figure S1. Visualization of cells distribution in GSE81608 and GSE86469 among clusters at different resolutions through K-means clustering algorithm. A GSE81608, the resolution is 0.7 was considered as a cutoff. B GSE86469, the resolution is 0.8 was considered as a cutoff. Figure S2. Expressions of the five DEMGs in the three cell types over trajectory time. Trend in expressions of five DEMGs during differentiation of three cell types over trajectory time. Figure S3. Expression of CDKN1C and DLK1 in different cell clusters of non-diabetic and T2DM samples. A Expression of CDKN1C in different cell clusters of non-diabetic and T2DM samples. B Expression of DLK1 in different cell clusters of non-diabetic and T2DM samples. Figure S4. Expression of CDKN1C and DLK1 in the major endocrine clusters, acinar cell and ductal cell of non-diabetic and T2DM samples. A The expression level of CDKN1C and DLK1 in major pancreatic cells of non-diabetic and T2DM patients. B The t-SNE plot of CDKN1C and DLK1 expressed in endocrine cells and exocrine cells. Figure S5. Relevance analysis between DEMGs and patient age. A Relevance analysis between CDKN1C and patient age. B Relevance analysis between DLK1 and patient age.
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Yang, T., Yan, C., Yang, L. et al. Identification and validation of core genes for type 2 diabetes mellitus by integrated analysis of single-cell and bulk RNA-sequencing. Eur J Med Res 28, 340 (2023). https://doi.org/10.1186/s40001-023-01321-1